Abstract
A capillary electrophoresis method was developed to determine the impurity of etodolac enantiomers. (2-Hydroxypropyl)-β-cyclodextrin (HP-β-CD) was used as a chiral selector and ketoprofen as an internal standard to improve the peak area precision. The seperation of the etodolac enantiomers was achived within 35 min at 15°C and its highest resolution was about 4.0 using phosphate buffer (0.1 M, pH 6.0) with 15 mM HP-β-CD and UV detection at 225 nm with a reference wavelength at 360 nm. This method allowed determination of 0.2% of (R)-(−)-etodolac in (S)-(+)-etodolac and method validation showed adequate linearity over the required range.
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Ali, I. and Aboul-Enein, H. Y., Enantioseparation of some clinically used drugs by HPLC using cellulose Tris (3,5-dichlorophenylcarbamate) chiral stationary phase. Biomed. Chromatogr., 17, 113–117 (2003).
Brocks, D. R., Jamali, F., Russell, A. S., and Skeith, D. J., J., The stereoselective pharmacokinetics of etodolac in young and elderly subjects, and after cholecystectomy. Clin. Pharmacol., 32, 982–989. (1992).
Brocks, D. R. and Jamali, F., Etodolac clinical pharmacokinetics. Clin. Pharmacokinetics, 26, 259–274 (1994).
Beckerscharfenkamp, U. and Blaschke, G., Evaluation of the steroselective metabolism of the chiral analgesic drug etodolac by high-performance liquid chromatography. J. Chromatogr. B, 621, 199–207 (1993).
Blaschke, G. and Chankvetadze, B., Enantiomer separation of drugs by capillary electromigration techniques. J. Chromatogr. A, 875, 3–25 (2000).
Brenna, E., Fuganti, C., Fuganti, D., Grasselli, P., Malpezzi, L., and Pedrocchi-Fantoni, G., New enzymatic and chemical approaches to enantiopure etodolac. Tetrahedron, 53, 17769–17780 (1997).
Chankvetadze, B., John Wiley and Sons., Capillary Electrophoresis in Chiral Analysis, (1997).
Fanali, S., Identification of chiral drug isomers by capillary electrophoresis. J. Chromatogr. A, 735, 77–121 (1996).
Fanali, S., Desiderio, C., Schulte, G., Heitmeier., Strickmann, D., Chankvetadze, B., and Blaschke, G., Chiral capillary electrophoresis-electrospray mass spectrometry coupling using vancomycin as chiral selector. J. Chromatogr. A, 800, 69–76 (1998).
Fanali, S., Enantioselective determination by capillary electrophoresis with cyclodextrins as chiral selectors. J. Chromatogr. A, 875, 89–122 (2000).
ICH Harmonnised Tripartite Guideline. Validation of Analytical Procedures Text and Methodology Q2(R1)., November 2005.
Jenson, M., Engert, A., Goebeler, M. E., Weissinger, F., Wilhelm, M., Osterborg, A., Knauf, W., Kimby, E. K., Rosen, P. J., Poynton, C., Rummel, M. J., Hillmen, P., Oliff, P., Kennedy, B., Jullusson, G., Latven, L., Scranton, S. A., and Paradiso, L. J., A randomized controlled phase III trial of recombinant human granulocyte colony-stimulating factor (filgrastim) for treatment of severe chronic neutropenia. Blood, 102, 2496 (2003).
Kim, K. R., La, S., Kim, J., Kim, J. H., and Goto, J., Simultaneous chiral discrimination of multiple profens by cyclodextrin-modified capillary electrophoresis in normal and reversed polarity modes. Electrophoresis, 24, 2407–2732 (2003).
Mayer, S. and Schurig, V., J. Liq. Enantiomer Separation by Electrochromatography in Open Tubular Columns Coated with Chirasil-Dex. J. Liq. Chromatogr., 16, 915–931 (1993).
Mignot, I., Presle, N., Lapicque, F., Monot, C., Dropsy, R., and Netter, P., Albumin binding sites for etodolac enantiomers. Chirality, 8, 271–280. (1996).
Nishi, H. and Terabe, S., Optical resolution of drugs by capillary electrophoretic techniques. J. Chromatogr. A, 694, 245 (1995).
Pablos, R. R., Ruiz, C. G., Crego, A. L., and Marina, L. M., Separation of etodolac enantiomers by capillary electrophoresis. Validation and application of the chiral method to the analysis of commercial formulations. Electrophoresis, 26 1106–1113 (2005).
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Dung, P.T., Ko, M.Y., Choi, H.J. et al. Determination of enantiomeric impurity of etodolac by capillary electrophoresis using (2-hydroxypropyl)-β-cyclodextrin. Arch. Pharm. Res. 31, 1218–1223 (2008). https://doi.org/10.1007/s12272-001-1292-4
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DOI: https://doi.org/10.1007/s12272-001-1292-4