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Protection of prenylated flavonoids from mori cortex radicis (Moraceae) against nitric oxide-induced cell death in neuroblastoma SH-SY5Y cells

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Abstract

Seven prenylated flavanoids, licoflavone C (1), cyclomulberrin (2), neocyclomorusin (3), sanggenon I (4), morusin (5), kuwanon U (6) and kuwanon E (7), and three 2-arylbenzofurans, moracin P (8), moracin O (9), and mulberrofuran Q (10) were isolated from the MeOH extract of Mori Cortex Radicis. Among these, compounds 2–7 enhanced cell viability in a dose-dependent manner against sodium nitroprusside-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay (EC50 values of 4.4, 5.6, 8.0, 6.4, 8.7, and 11.9 μg/mL, respectively). Among 10 compounds, C-3 prenylated flavones (2, 3, and 5) and prenylated flavanones (4, 6, and 7) showed cell protection. However, compound 1 which lacks the prenyl group at C-3 and three 2-arylbenzofurans (8–10) did not show protective effect. The order of cell protection was as follow: C-3 prenylated flavones (2, 3, and 5) > prenylated flavanones (4, 6, and 7) > 2-arylbenzofurans (8–10) and flavone (1). From this result, we show that some prenylated flavones and flavanones might protect neuronal cells against nitrosative stress-mediated cell death. Even though further evaluations are necessary in vitro and in vivo study, we carefully suggest that some prenylated flavonoids from Mori Cortex Radicis might protect neuronal cells from neurodegenerative diseases.

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Authors and Affiliations

  1. Korea Forest Research Institute, Seoul, 130-712, Korea

    Hak Ju Lee

  2. Natural Product Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, Korea

    Da Hyun Lyu, Uk Koo, Kung-Woo Nam & Woongchon Mar

  3. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Korea

    Seong Su Hong, Kem Ok Kim & Dongho Lee

  4. College of Pharmacy, Kangwon National University, Chuncheon, 200-701, Korea

    Kyeong Ho Kim

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  1. Hak Ju Lee
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Correspondence to Dongho Lee or Woongchon Mar.

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Lee, H.J., Lyu, D.H., Koo, U. et al. Protection of prenylated flavonoids from mori cortex radicis (Moraceae) against nitric oxide-induced cell death in neuroblastoma SH-SY5Y cells. Arch. Pharm. Res. 35, 163–170 (2012). https://doi.org/10.1007/s12272-012-0118-7

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