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Triterpenoid saponins from the roots of Rosa rugosa Thunb. as rat intestinal sucrase inhibitors

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Abstract

Medicinal plants constitute an important source of potential therapeutic agents for diabetes. The purpose of present study is to investigate the effect of root extract of Rosa rugosa Thunb. on inhibition of sucrase related to diabetes mellitus (DM). Bioassay-guided fractionation of the methanol extract led to the identification of 13 triterpenoid saponins (1–13). Their structures were elucidated on the basis of extensive spectroscopic analysis, including 1D, 2D NMR, and MS. The n-butanol fraction showed potent rat intestinal sucrase inhibitory activity with value of 87.62 ± 5.84 % inhibition compared to the positive control acarbose (50.96 ± 2.97 % inhibition at 0.02 mM). Subsequently, compounds 11–13 (1.0 mM) exhibited significant sucrase inhibitory activity, with inhibition percentage values of 41.17 ± 3.52, 46.80 ± 4.00, and 39.39 ± 4.19 %, respectively. Whereas, compounds 2–6, 8, and 10 showed moderate sucrase inhibitory activity (ranging from 13.26 ± 7.00 to 32.08 ± 6.04 % inhibition) at a same concentration. The data provide a starting point for creating new sucrase inhibitors, which may be useful for the development of effective therapies for the treatment of DM.

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Acknowledgments

This work was supported by a grant from the Priority Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2009-0093815), Republic of Korea.

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Authors and Affiliations

  1. College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Nguyen Phuong Thao, Bui Thi Thuy Luyen & Young Ho Kim

  2. Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam

    Nguyen Phuong Thao, Bui Thi Thuy Luyen, Tran Manh Hung, Nguyen Xuan Cuong, Nguyen Hoai Nam & Chau Van Minh

  3. Department of Food and Nutrition, Hannam University, Daejeon, 305-811, Republic of Korea

    Sung Hoo Jo & Young In Kwon

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  1. Nguyen Phuong Thao
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Correspondence to Young Ho Kim.

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Thao, N.P., Luyen, B.T.T., Jo, S.H. et al. Triterpenoid saponins from the roots of Rosa rugosa Thunb. as rat intestinal sucrase inhibitors. Arch. Pharm. Res. 37, 1280–1285 (2014). https://doi.org/10.1007/s12272-014-0384-7

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  • DOI: https://doi.org/10.1007/s12272-014-0384-7

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