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Regulation of sugar metabolism in rice (Oryza sativa L.) seedlings under arsenate toxicity and its improvement by phosphate

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Abstract

The effect of arsenate with or without phosphate on the growth and sugar metabolism in rice seedlings cv. MTU 1010 was studied. Arsenate was found to be more toxic for root growth than shoot growth and water content of the seedlings gradually decreased with increasing concentrations. Arsenate exposure at 20 μM and 100 μM resulted in an increase in reducing sugar content and decrease in non-reducing sugar content. There was a small increase in starch content, the activity of starch phosphorylase was increased but α-amylase activity was found to be decreased. Arsenate toxicity also affected the activities of different carbohydrate metabolizing enzymes. The activities of sucrose degrading enzymes viz., acid invertase and sucrose synthase were increased whereas, the activity of sucrose synthesizing enzyme, viz. sucrose phosphate synthase declined. The combined application of arsenate with phosphate exhibited significant alterations of all the parameters tested under the purview of arsenate treatment alone which was congenial to better growth and efficient sugar metabolism in rice seedlings. Thus, the use of phosphorus enriched fertilizers may serve to ensure the production of healthy rice plants in arsenic contaminated soils.

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Abbreviations

As:

arsenic

cv.:

cultivar

DNSA:

3,5-dinitrosalicylic acid

DTT:

dithiothreitol

EDTA:

ethylenediamine tetraacetic acid

fw:

fresh weight

HEPES:

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid

KI:

potassium iodide

PFD:

photon flux density

PMSF:

phenyl methyl sulphonyl fluoride

SE:

standard error

SPS:

sucrose phosphate synthase

SS:

sucrose synthase

TCA:

trichloroacetic acid

UDP:

uridine-di-phosphate

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Authors and Affiliations

  1. Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India

    Bhaskar Choudhury, Souvik Mitra & Asok K. Biswas

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  1. Bhaskar Choudhury
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  2. Souvik Mitra
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  3. Asok K. Biswas
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Correspondence to Asok K. Biswas.

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Choudhury, B., Mitra, S. & Biswas, A.K. Regulation of sugar metabolism in rice (Oryza sativa L.) seedlings under arsenate toxicity and its improvement by phosphate. Physiol Mol Biol Plants 16, 59–68 (2010). https://doi.org/10.1007/s12298-010-0008-8

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