Abstract
The Alternaria mycotoxin tenuazonic acid (TA) was quantified in fruit juices (n = 50), cereals (n = 12) and spices (n = 38) using a recently developed stable isotope dilution assay (SIDA). [13 C6,15 N]-TA was used as the internal standard. Method validation revealed low limits of detection (LODs) of 0.15 μg/kg (fruit juices), 1.0 μg/kg (cereals) and 17 μg/kg (spices). The respective limits of quantitation were about three times higher. Recovery was about 100% for all matrices. The precision (relative standard deviation of replicate analyses of naturally contaminated samples) was 4.2% (grape juice; 1.7 μg/kg), 3.5% (whole wheat flour; 36 μg/kg) and 0.9% (curry powder; 215 μg/kg). The median content of TA in the analyzed samples was 1.8 μg/kg (fruit juices), 16 μg/kg (cereals) and 500 μg/kg (spices). Positive samples amounted to 86% (fruit juices), 92% (cereals) and 87% (spices).
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References
Asam S, Konitzer K, Schieberle P, Rychlik M (2009) Stable isotope dilution assays of alternariol and alternariol monomethyl ether in beverages. J Agric Food Chem 57:5152–5160
Asam S, Liu Y, Konitzer K, Rychlik M (2011) Development of a stable isotope dilution assay for tenuazonic acid. J Agric Food Chem 59:2980–2987
Azcarate MP, Patriarca A, Terminiello L, Pinto VF (2008) Alternaria toxins in wheat during the 2004 to 2005 Argentinean harvest. J Food Prot 71:1262–1265
Boonzaaijer G, van Osenbruggen WA, Kleinnijenhuis AJ, van Dongen WD (2008) An exploratory investigation of several mycotoxins and their natural occurrence in flavour ingredients and spices, using al multi-mycotoxin LC-MS/MS method. World Mycotoxin J 1:167–174
Bottalico A, Logrieco A (1998) Toxigenic Alternaria species of economic importance. In: Kaushal K, Bhatnagar D (eds) Mycotoxins in Agriculture and Food Safety. Marcel Dekker, New York, pp 65–108
Brady OL, Elsmie GV (1926) The use of 2:4-dinitrophenylhydrazine as a reagent for aldehydes and ketones. Analyst 51:77–78
Combina M, Dalcero AM, Torres A (1998) Spectrometric studies on stability of tenuazonic acid (TeA) solution in organic solvents. Mycotoxin Res 14:54–59
Da Motta S, Soares LMV (2001) Survey of Brazilian tomato products for alternariol, alternariol monomethyl ether, tenuazonic acid and cyclopiazonic acid. Food Addit Contam 18:630–634
Fazekas B, Tar A, Kovacs M (2005) Aflatoxin and ochratoxin A content of spices in Hungary. Food Addit Contamin 22:856–863
Hernandez-Hierro JM, Garcia-Villanova RJ, Torrero PR, Toruno-Fonseca IM (2008) Aflatoxins and ochratoxin A in red paprika for retail sale in Spain: Occurrence and evaluation of a simultaneous analytical method. J Agric Food Chem 56:751–756
Lau BPY, Scott PM, Lewis D, Kanhere S, Cleroux C, Roscoe V (2003) Liquid chromatography-mass spectroscopy and liquid chromatography-tandem mass spectroscopy of Alternaria mycotoxins alternariol and alternariol monomethylether in fruit juices and beverages. J Chromatogr A 998:119–131
Li FQ, Yoshizawa T (2000) Alternaria Mycotoxins in Weathered Wheat from China. J Agric Food Chem 48:2920–2924
Miller FA, Rightsel WA, Sloan BJ, Ehrlich J, French JC, Bartz QR, Dixon GJ (1963) Antiviral Activity of tenuazonic acid. Nature 200:1338–1339
Mislivec PB, Bruce VR, Stack ME, Bandler R (1987) Molds and tenuazonic acid in fresh tomatoes used for catsup production. J Food Prot 50:38–41
Patriarca A, Azcarate MP, Terminiello L, Pinto VF (2007) Mycotoxin production by Alternaria strains from Argentinean wheat. Int J Food Microbiol 119:219–222
Pinto VF (2008) Detection and determination of Alternaria mycotoxins in fruits and vegetables. In: Barkai-Golan R, Paster N (eds) mycotoxins in fruits and vegetables, 1st edn. Elsevier, San Diego, pp 271–278
Rosett T, Sankhala RH, Stickings CE, Taylor MEU, Thomas R (1957) Studies in the biochemistry of micro-organisms. Metabolites of Alternaria Tenuis Auct.: Culture Filtrate Products. Biochem J 67:390–400
Rychlik M, Asam S (2008) Stable isotope dilution assays in mycotoxin analysis. Anal Bioanal Chem 390:617–628
Santos L, Marín S, Sanchis V, Ramos AJ (2010) Co-occurrence of aflatoxins, ochratoxin A and zearalenone in Capsicum powder samples available on the Spanish market. Food Chem 122:826–830
Scott PM (2001) Analysis of agricultural commodities and foods for Alternaria mycotoxins. J AOAC Int 84:1809–1817
Scott PM, Kanhere SR (1980) Liquid chromatographic determination of tenuazonic acids in tomato paste. J Assoc Off Anal Chem 63:612–621
Scott PM, Lawrence GA, Lau BPY (2006) Analysis of wines, grape juices and cranberry juices for Alternaria toxins. Mycotoxin Res 22:142–147
Scott PM, Stoltz DR (1980) Mutagens produced by Alternaria alternata. Mutat Res 78:33–40
Shephard GS, Thiel PG, Sydenham EW, Vleggaar R, Marasas WFO (1991) Reversed-phase high-performance liquid chromatography of tenuazonic acid and related tetramic acids. J Chromatogr Biomed Appl 566:195–205
Shigeura HT, Gordon CN (1963) The biological activity of tenuazonic acid. Biochemistry 2:1132–1137
Siegel D, Merkel S, Koch M, Nehls I (2010) Quantification of the Alternaria mycotoxin tenuazonic acid in beer. Food Chem 120:902–906
Siegel D, Rasenko T, Koch M, Nehls I (2009) Determination of the Alternaria mycotoxin tenuazonic acid in cereals by high-performance liquid chromatography–electrospray ionization ion-trap multistage mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. J Chromatogr A 1216:4582–4588
Smith ER, Fredrickson TN, Hadidian Z (1968) Toxic effects of the sodium and the N, N'-dibenzylethylenediamine salts of tenuazonic acid (NSC-525816 and NSC-82260). Cancer Chemother Rep 52:579–585
Stack ME, Mislivec PB, Roach JAG, Pohland AE (1985) Liquid chromatographic determination of tenuazonic acid and alternariol methyl ether in tomatoes and tomato products. J Assoc Off Anal Chem 68:640–642
Steyn PS, Rabiet CJ (1976) Characterization of magnesium and calcium tenuazonate from Phoma Sorghina. Phytochemistry 15:1977–1979
Stickings CE (1959) Studies in the biochemistry of micro-organisms. Metabolites of Alternaria Tenuis Auct.: the structure of tenuazonic acid. Biochem J 72:332–340
Terminiello L, Patriarca A, Pose G, Pinto VF (2006) Occurrence of alternariol, alternariol monomethyl ether and tenuazonic acid in Argentinean tomato puree. Mycotoxin Res 22:236–240
Umetsu N, Kaji J, Aoyama K, Tamari K (1974) Toxins in blast-diseased rice plants. Agric Biol Chem 38:1867–1874
Umetsu N, Kaji J, Tamari K (1972) Investigation on the toxin production by several blast fungus strains and isolation of tenuazonic acid as a novel toxin. Agric Biol Chem 36:859–866
Vogelgesang J, Hädrich J (1998) Limits of detection, identification and determination: a statistical approach for practitioners. Accred Qual Assur 3:242–255
Webley DJ, Jackson KL, Mullins JD, Hocking AD, Pitt JI (1997) Alternaria toxins in weather-damaged wheat and sorghum in the 1995–1996 Australian harvest. Aust J Agric Res 48:1249–1255
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Asam, S., Lichtenegger, M., Liu, Y. et al. Content of the Alternaria mycotoxin tenuazonic acid in food commodities determined by a stable isotope dilution assay. Mycotoxin Res 28, 9–15 (2012). https://doi.org/10.1007/s12550-011-0111-x
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DOI: https://doi.org/10.1007/s12550-011-0111-x