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Effects of a probiotic bacterial Lactobacillus rhamnosus dietary supplement on the crowding stress response of juvenile Nile tilapia Oreochromis niloticus

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  • Aquaculture
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Abstract

To test the effects of probiotic bacteria against crowding stress, juvenile Nile tilapia Oreochromis niloticus were fed commercial feed supplemented with 1 × 1010 cfu/g pellets of Lactobacillus rhamnosus for 3 weeks. The fish were confined and subjected to crowding for 7 and 14 days after which their physiological condition was investigated. The administration of probiotic prophylaxis improved growth performance even in the stressed fish. After 7 days of stress exposure, fish receiving probiotic-supplemented feed showed proactive behavior and coping responses to the stressor, as evidenced by elevated plasma glucose levels and osmolality, stabilized plasma electrolytes, and a higher RNA:DNA ratio. Fish stressed for 7 days and fed normal commercial diet showed impairment of plasma electrolytes; after 14 days, the plasma osmolality, electrolytes, glucose, and RNA:DNA ratio decreased, indicating physiological maladaptation to the stressor. The growth rate was also found to be reduced, suggesting that energy demand exceeded the energy available from metabolism and dietary uptake. This latter effect was not observed in fish fed the probiotic-supplemented diet. The results suggest that probiotic bacteria prophylaxis may increase energy availability for metabolic support of the crowding stress response and improve the stress coping capacity of fish.

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Authors and Affiliations

  1. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-Ku, Tokyo, 108-8477, Japan

    Ana Teresa Gonçalves, Masashi Maita, Kunihiko Futami & Takayuki Katagiri

  2. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-Ku, Tokyo, 108-8477, Japan

    Masato Endo

Authors
  1. Ana Teresa Gonçalves
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  2. Masashi Maita
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  3. Kunihiko Futami
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  4. Masato Endo
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  5. Takayuki Katagiri
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Correspondence to Takayuki Katagiri.

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Gonçalves, A.T., Maita, M., Futami, K. et al. Effects of a probiotic bacterial Lactobacillus rhamnosus dietary supplement on the crowding stress response of juvenile Nile tilapia Oreochromis niloticus . Fish Sci 77, 633–642 (2011). https://doi.org/10.1007/s12562-011-0367-2

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  • DOI: https://doi.org/10.1007/s12562-011-0367-2

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