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Contamination of seeds of Iceland poppy (Papaver nudicaule L.) by Fusarium oxysporum

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Abstract

In 2011, a new Fusarium wilt of Papaver nudicaule was observed in a commercial nursery near Ventimiglia (Imperia province, northern Italy) as well as in the Regional Institute of Floriculture (I.R.F.) of Sanremo (Imperia province, northern Italy). Molecular analysis enabled identification of the causal agent as a new forma specialis called Fusarium oxysporum f. sp. papaveris. The origin of the infection was found on P. nudicaule seeds. However, disinfection with sodium hypochlorite was able to eliminate the pathogen infection. Virulence of ten isolates obtained from seeds was evaluated by pathogenicity assay. The more virulent strains were analyzed by phylogenetic analysis on the basis of the EF-1α, pg1 and pgx4 genes. Sequences obtained by PCR amplification were aligned with other formae speciales of Fusarium oxysporum from the GenBank and used in the construction of the phylogenetic trees. Seed infections have been observed in the case of many vegetable crops; however, this phenomenon has been less studied in the case of ornamental crops. As a consequence of seed exchanges or transmission via infected seeds, new and old pathogenic species of Fusarium are continuously introduced into new areas and production systems.

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Acknowledgments

The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 311875.

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Authors and Affiliations

  1. AGROINNOVA, Università di Torino, 10095, Grugliasco, Italy

    Domenico Bertetti, Giuseppe Ortu, Maria Lodovica Gullino & Angelo Garibaldi

  2. DISAFA, Università di Torino, 10095, Grugliasco, Italy

    Maria Lodovica Gullino

Authors
  1. Domenico Bertetti
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  2. Giuseppe Ortu
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  3. Maria Lodovica Gullino
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  4. Angelo Garibaldi
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Correspondence to Giuseppe Ortu.

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Bertetti, D., Ortu, G., Gullino, M.L. et al. Contamination of seeds of Iceland poppy (Papaver nudicaule L.) by Fusarium oxysporum . Phytoparasitica 43, 189–196 (2015). https://doi.org/10.1007/s12600-014-0431-4

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  • DOI: https://doi.org/10.1007/s12600-014-0431-4

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