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Hemato-Immunological Responses and Disease Resistance in Siberian Sturgeon Acipenser baerii Fed on a Supplemented Diet of Lactobacillus plantarum

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Abstract

A feeding trial was conducted to investigate the effects of different levels of dietary Lactobacillus plantarum on hemato-immunological parameters and resistance against Streptococcus iniae infection in juvenile Siberian sturgeon Acipenser baerii. Fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with L. plantarum at different concentrations [1 × 107, 1 × 108 and 1 × 109 colony-forming units (cfu) g−1] and a control (non-supplemented basal) diet for 8 weeks. Innate immune responses (immunoglobulin (Ig), alternative complement activity (ACH50) and lysozyme activity) were significantly higher in fish fed the 1 × 108 and 1 × 109 cfu g−1 L. plantarum diet compared to the other groups (P < 0.05). Furthermore, fish fed on various levels of L. plantarum significantly showed higher red blood cell (RBC), hemoglobin (Hb), white blood cell (WBC) and monocyte compared to those of the control group (P < 0.05). At the end of the feeding experiment, some fish were challenged with S. iniae to quantify the level of disease resistance. The mortality after S. iniae challenge was decreased in fish fed a probiotic. These results indicated that dietary supplementation of L. plantarum improved immune response and disease resistance of Siberian sturgeon juvenile.

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Authors and Affiliations

  1. Caspian Sea International Sturgeon Research Institute, Rasht, Iran

    Moheb Ali Pourgholam & Mohammad Ali Yazdani Sadati

  2. Fisheries Department, Faculty of Natural Resources, Islamic Azad University, Lahijan Branch, Lahijan, Iran

    Hossein Khara

  3. Ecology Research Institute of the Caspian Sea, Sari, Iran

    Reza Safari

  4. Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran

    Mohammad Sadegh Aramli

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  1. Moheb Ali Pourgholam
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  2. Hossein Khara
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Correspondence to Mohammad Sadegh Aramli.

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Moheb Ali Pourgholam, Hossein Khara, Reza Safari, Mohammad Ali Yazdani Sadati and Mohammad Sadegh Aramli declares that they have no conflict of interest.

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All fish manipulations were conducted in accordance with the guidelines on the care and use of animals for scientific purposes (National Health and Medical Research Council, Australia).

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Pourgholam, M.A., Khara, H., Safari, R. et al. Hemato-Immunological Responses and Disease Resistance in Siberian Sturgeon Acipenser baerii Fed on a Supplemented Diet of Lactobacillus plantarum . Probiotics & Antimicro. Prot. 9, 32–40 (2017). https://doi.org/10.1007/s12602-016-9229-7

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