Skip to main content
SpringerLink
Log in

Transmission Near-Field Scanning Optical Microscopy Investigation on Cellular Uptake Behavior of Iron Oxide Nanoparticles

  • Published:
BioNanoScience Aims and scope Submit manuscript

Abstract

Cellular uptake behavior of iron oxide nanoparticles is investigated using a transmission near-field scanning optical microscopy (NSOM) without the need of fluorescent labeling. By using the transmission NSOM system, we could simultaneously explore the near-field optical analysis of the cell interior and record the topographic information of the cell surface. The cell endocytosis of iron oxide nanoparticles by normal breast MCF10A cells is first studied by this transmission NSOM system, and this dual functional nanoscale-resolution microscopy shows the capability of mapping the spatial localization of nanoparticles in/outside cell surface without the need of fluorescence labeling. Nanoscale optical signature patterns for iron oxide nanoparticle-loaded vesicles inside the cells were observed and analyzed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Pawley, J. B. (Ed.). (1995). Handbook of biological confocal microscopy. New York: Plenum.

    Google Scholar 

  2. Zenobi, R. (2008). Analytical and Bioanalytical Chemistry, 390, 215.

    Article  Google Scholar 

  3. Betzig, E., & Trautman, J. K. (1992). Science, 257, 189.

    Article  Google Scholar 

  4. de Lange, F., Cambi, A., Huijbens, R., de Bakker, B., Rensen, W., Garcia-Parajo, M., van Hulst, N., Figdor, C. G. (2001). Journal of Cell Science, 114, 4153.

    Google Scholar 

  5. Enderle, T., Ha, T., Chemla, D. S., Weiss, S. (1998). Ultramicroscopy, 71, 303.

    Article  Google Scholar 

  6. Hwang, J., Gheber, L. A., Margolis, L., Edidin, M. (1998). Biophysical Journal, 74, 2184.

    Article  Google Scholar 

  7. Ianoul, A., Street, M., Grant, D., Pezacki, J., Taylor, R. S., Johnston, L. J. (2004). Biophysical Journal, 87, 3525.

    Article  Google Scholar 

  8. Koopman, M., Cambi, A., de Bakker, B. I., Joosten, B., Figdor, C. G., van Hulst, N. F., Garcia-Parajo, M. F. (2004). FEBS Letters, 573, 6.

    Article  Google Scholar 

  9. Thurn, K. T., Paunesku, T., Wu, A., Brown, E. M., Lai, B., Vogt, S., Maser, J., Aslam, M., Dravid, V., Bergan, R., Woloschak, G. E. (2009). Small, 5, 1318.

    Article  Google Scholar 

  10. Maxwell, D. J., Bonde, J., Hess, D. A., Hohm, S. A., Lahey, R., Zhou, P., Creer, M. H., Piwnica Worms, D., Nolta, J. A. (2008). Stem Cells, 26, 517.

    Article  Google Scholar 

  11. H. Mader, X. H. Li, S. Saleh, M. Link, P. Kele, O. S.Wolfbeis. Fluorescence methods and applications: Spectroscopy, imaging, and probes. Wiley-Blackwell. 218 (2008).

  12. Lee, J. H., Schneider, B., Jordan, E. K., Liu, W., Frank, J. A. (2008). Advanced Materials, 20, 2512.

    Article  Google Scholar 

  13. Clark, P. R., Chua-Anusorn, W., St Pierre, T. G. (2003). Magnetic Resonance in Medicine, 49, 572.

    Article  Google Scholar 

  14. Gupta, A. K., & Gupta, M. (2005). Biomaterials, 26, 3995.

    Article  Google Scholar 

  15. Hergt, R., Andra, W., d’Ambly, C. G., Hilger, I., Kaiser, W. A., Richter, U., Schmidt, H. G. (1998). IEEE Transactions in Magnetics, 34, 3745.

    Article  Google Scholar 

  16. Lee, H., Lee, E., Kim, D. K., Jang, N. K., Jeong, Y. Y., Jon, S. (2006). Journal of the American Chemical Society, 128, 7383.

    Article  Google Scholar 

  17. Frankel, D. J., Pfeiffer, J. R., Surviladze, Z., Johnson, A. E., Oliver, J. M., Wilson, B. S., Burns, A. R. (2006). Biophysical Journal, 90, 2404.

    Article  Google Scholar 

  18. Stark, M., Moller, C., Muller, D. J., Guckenberger, R. (2001). Biophysical Journal, 80, 3009.

    Article  Google Scholar 

  19. Chithrani, B. D., Ghazani, A. A., Chan, W. C. W. (2006). Nano Letters, 6, 662.

    Article  Google Scholar 

  20. Wilhelm, C., Gazeau, F., Roger, J., Pons, J. N., Bacri, J. C. (2002). Langmuir, 18, 8148.

    Article  Google Scholar 

  21. G.M. Cooper (Ed.). The cell: A molecular approach. ASM Press, Washington D.C. (2000)

  22. Wilhelm, C., Billotey, C., Roger, J., Pons, J. N., Bacri, J. C., Gazeau, F. (2003). Biomaterials, 24, 1001.

    Article  Google Scholar 

  23. Jin, H., Heller, D. A., Sharma, R., Strano, M. S. (2009). ACS Nano, 3, 149.

    Article  Google Scholar 

Download references

Acknowledgment

We gratefully acknowledge the Center of Excellence of Nanotechnology for Treatment, Understanding, and Monitoring of Cancer (NANOTUMOR) for providing financial support for this project. Mr. Stephen McDaniel in the Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of California is gratefully acknowledged for assistance in preparing the microtome cell slides for TEM.

Author information

Authors and Affiliations

  1. Department of Mechanical Engineering, University of California Riverside, Riverside, CA, 92521, USA

    Yu Zhang, Vahid Yazdanpanah & Cengiz S. Ozkan

  2. Institute of Textiles and Clothing, the Hong Kong Polytechnic University, Hong Kong, China

    Yu Zhang & Yi Li

  3. Department of Electrical Engineering, University of California Riverside, Riverside, CA, 92521, USA

    Jennifer Reiber Kyle, Miro Penchev & Mihrimah Ozkan

  4. Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China

    Jinjiang Yu & Mo Yang

  5. Nanomedicine Research Center, Gazi University, Besevler, Ankara, Turkey

    Gurer Budak

  6. Nanotechnology Research Center, Department of Physics, Bilkent University, Ankara, Turkey

    Ekmel Ozbay

Authors
  1. Yu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jennifer Reiber Kyle
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miro Penchev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vahid Yazdanpanah
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jinjiang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mo Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gurer Budak
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ekmel Ozbay
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Mihrimah Ozkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Cengiz S. Ozkan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cengiz S. Ozkan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Y., Kyle, J.R., Penchev, M. et al. Transmission Near-Field Scanning Optical Microscopy Investigation on Cellular Uptake Behavior of Iron Oxide Nanoparticles. BioNanoSci. 2, 135–143 (2012). https://doi.org/10.1007/s12668-012-0043-8

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12668-012-0043-8

Keywords

Search

Navigation