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Biofabrication of Silver Nanoparticles from Diospyros montana, Their Characterization and Activity Against Some Clinical Isolates

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Abstract

Very small-sized silver nanoparticles (Ag-NPs) have been synthesized from dried leaf extract of Diospyros montana in aqueous medium. They have been characterized by various spectroscopic techniques (UV-Vis, FTIR spectroscopy), SEM, TEM, and EDX analyses. Particle size has been analyzed by Zetasizer. Their antibacterial screening has also been done against nine pathogenic bacterial strains. Formation of Ag-NPs was confirmed from a sharp absorption peak at 421 nm in the UV-Vis region of the spectrum. Its FTIR spectrum showed sharp ʋ(C=O) at 1635 cm−1 due to the presence of quinonoid type molecule in the extract which undergoes decrease in intensity after NP formation. Although three types of NPs were detected, their average diameter was found to be 61.69 nm. The particles of largest size were in abundance. However, from TEM analysis, five different types of NPs varying in shape and size (7.44, 13.10, 18.20, 8.90, and 10.60 nm) have been detected. SEM analysis showed that NPs were irregular in shape. EDX analysis showed sharp signal for Ag-NPs which suggest their crystalline structure. Their antibacterial activity was done on nine different bacterial strains using agar well and disk diffusion method according to CLSI guidelines. Maximum activity of Ag-NPs was observed against Klebsiella pneuomoniae and Escherichia coli while moderate efficacy was seen in other bacteria. Resistant pattern was also observed against Streptococcus viridans and S. mutans. Our study suggests that Ag-NPs can act as an antibacterial agent.

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References

  1. Husen, A., & Siddiqi, K. S. (2014). Phytosynthesis of nanoparticles: Concept, controversy and application. Nanoscale Research Letters, 9, 229.

    Article  Google Scholar 

  2. Dakal, T. C., Kumar, A., Majumdar, R. S., & Yadav, V. (2016). Mechanistic basis of antimicrobial actions of silver nanoparticles. Frontiers in Microbiology, 7, 1831.

    Article  Google Scholar 

  3. Siddiqi, K. S., Husen, A., & Rao, R. A. K. (2018). A review on biosynthesis of silver nanoparticles and their biocidal properties. Journal of Nanbiotechnology, 16, 14.

    Article  Google Scholar 

  4. Miller, L. P., & McCallan, S. E. A. (1957). Toxic action of metal ions to fungus spores. Journal of Agricultural and Food Chemistry, 5, 116122.

    Article  Google Scholar 

  5. Rayman, M. K., Lo, T. C., & Sanwal, B. D. (1972). Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations. The Journal of Biological Chemistry, 247, 6332–6339.

    Google Scholar 

  6. Schreurs, W. J., & Rosenberg, H. (1982). Effect of silver ions on transport and retention of phosphate by Escherichia coli. Journal of Bacteriology, 152, 7–13.

    Google Scholar 

  7. Siddiqi, K. S., & Husen, A. (2016). Fabrication of metal nanoparticles from fungi and metal salts: Scope and application. Nanoscale Research Letters, 11, 98.

    Article  Google Scholar 

  8. Siddiqi, K. S., & Husen, A. (2016). Fabrication of metal and metal oxide nanoparticles by algae and their toxic effects. Nanoscale Research Letters, 11, 363.

    Article  Google Scholar 

  9. Gandhi, H., & Khan, S. (2016). Biological synthesis of silver nanoparticles and its antibacterial activity. Journal of Nanoscience and Nanotechnology, 7, 366.

    Google Scholar 

  10. Raffi, M., Hussain, F., Bhatti, T. M., Akhter, J. I., Hameed, A., & Hasan, M. M. (2008). Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224. Journal of Materials Science and Technology, 24, 192–196.

    Google Scholar 

  11. Muthukrishnan, S., Bhakya, S., Kumar, T. S., & Rao, M. V. (2015). Biosynthesis, characterization and antibacterial effect of plant-mediated silver nanoparticles using Ceropegia thwaitesuii—An endemic species. Industrial Crops and Products, 63, 119–124.

    Article  Google Scholar 

  12. Catalina, M. J., & Hoek, E. M. V. (2010). A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. Journal of Nanoparticle Research, 12, 1531–1551.

    Article  Google Scholar 

  13. Richards, R., Odelola, M. E., & Anderson, B. (1984). Effect of silver on whole cells and spheroplasts of a silver resistant Pseudomaonas aeruginosa. Microbes, 39, 151–157.

    Google Scholar 

  14. Marini, M., De Niederhausen, N., Iseppi, R., Bondi, M., Sabia, C., Toselli, M., & Pilati, F. (2007). Antibacterial activity of plastic coated with silver doped organic-inorganic hybrid coatings prepared by sol-gel process. Biomacromolecules, 8, 1246–1254.

    Article  Google Scholar 

  15. Kanipandian, N., Kannan, S., Ramesh, R., Subramanian, P., & Thirumurugan, R. (2014). Characterization, antioxidant and cytotoxicity evaluation of green synthesized silver nanoparticles using Cleistanthus collinus extract as surface modifier. Materials Research Bulletin, 49, 494–502.

    Article  Google Scholar 

  16. Latha, M., Priyanka, M., Rajasekar, P., Manikandan, R., & Prabhu, N. M. (2016). Biocompatibility and antibacterial activity of the Adathoda vasica Linn. extract mediated silver nanoparticles. Microbial Pathogenesis, 93, 88–94.

    Article  Google Scholar 

  17. Li, W. R., Xie, X. B., Shi, Q. S., Zeng, H. Y., Qu Yang, Y. S., & Chen, Y. B. (2010). Antibacterial activity and mechanism of silver nanoparticles in E. Coli. Applied Microbiology and Biotechnology, 85, 1115–1122.

    Article  Google Scholar 

  18. Lee, J. H., Lim, J. M., Velmurugan, P., Park, Y. J., Park, Y. J., Bang, K. S., & Oh, B. T. (2016). Photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity. Journal of Photochemistry and Photobiology B: Biology, 162, 93–99.

    Article  Google Scholar 

  19. Rajakumar, G., Gomathi, T., Thiruvengadam, M., Rajeswari, V. D., Kalbana, V. N., & Chung, I.-M. (2017). Evaluation of anti cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract. Microbial Pathogenesis, 103, 123–128.

    Article  Google Scholar 

  20. Husen, A., & Siddiqi, K. S. (2014). Plants and microbes assisted selenium nanoparticles: Characterization and application. Journal of Nanbiotechnology, 12, 28.

    Article  Google Scholar 

  21. Siddiqi, K. S., & Husen, A. (2016). Green synthesis, characterization and uses of palladium/platinum nanoparticles. Nanoscale Research Letters, 11, 482.

    Article  Google Scholar 

  22. Siddiqi, K. S., & Husen, A. (2017). Recent advances in plant-mediated engineered gold nanoparticles and their application in biological system. Journal of Trace Elements in Medicine and Biology, 40, 10–23.

    Article  Google Scholar 

  23. Siddiqi, K. S., Rahman, A., & Tajuddin, H. A. (2016). Biogenic fabrication of iron/iron oxide nanoparticles and their application. Nanoscale Research Letters, 11, 498.

    Article  Google Scholar 

  24. Husen, A. (2017). Gold nanoparticles from plant system: Synthesis, characterization and their application. In M. Ghorbanpourn, K. Manika, & A. Varma (Eds.), Nanoscience and plant–soil systems (Vol. 48, pp. 455–479). Cham: Springer International Publishing AG.

    Chapter  Google Scholar 

  25. Siddiqi, K. S., Rashid, M., Rahman, A., Tajuddin, H. A., & Rehman, S. (2018). Biogenic fabrication and characterization of silver nanoparticles using aqueous-ethanolic extract of lichen (Usnea longissima) and their antimicrobial activity. Biomaterial Research, 22, 23.

    Article  Google Scholar 

  26. Mishra, V. K., Husen, A., Rahman, Q. I., Iqbal, M., Sohrab, S. S., Yassin, M. O., & Bachheti, R. K. (2019). Plant-based fabrication of silver nanoparticles and their application. In A. Husen & M. Iqbal (Eds.), Nanomaterials and plant potential (pp. 135–175). Cham: Springer International Publishing AG.

    Chapter  Google Scholar 

  27. Vivek, R., Thangam, R., Muthuchelian, K., Gunasekaran, P., Kaveri, K., & Kannan, S. (2012). Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract it’s in vitro cyctotoxic effect on MCF-7 cells. Process Biochemistry, 47, 2405–2410.

    Article  Google Scholar 

  28. Loo, Y. Y., Chieng, B. W., Nishibuchi, M., & Radu, S. (2012). Synthesis of silver nanoparticles by using tea leaf extract from Camellia Sinensis. International Journal of Nanomedicine, 7, 4263–4267.

    Google Scholar 

  29. Mehta, B. K., Chhajlani, M., & Shrivastava, B. D. (2017). Green synthesis of silver nanoparticles and their characterization by XRD. Journal of Physics: Conference Series, 836, 012050.

    Google Scholar 

  30. Salehi, S., Shandiz, S. A., Ghanbar, F., Darvish, M. R., Ardestani, M. S., Mirzaie, A., & Jafari, M. (2016). Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. International Journal of Nanomedicine, 11, 1835–1846.

    Google Scholar 

  31. Anonymous (2013) The wealth of India, first supplement series. CSIR- national institute of science communication and informative resources. Dr K.S. Krishanan Marg, New Delhi Vol. 3: D-I, pp. 28.

  32. O’ Brien, P. J. (1991). Molecular mechanisms of quinone cytotoxicity. Chemico-Biological Interactions, 80, 1–41.

    Article  Google Scholar 

  33. Chakrabarti, S., Roy, M., Hazra, R., & Bhattacharya, R. K. (2002). Induction of apoptosis in human cancer cell lines by diospyrin, a plant derived bis-naphthoquinoid and its synthetic derivatives. Cancer Letters, 188, 85–93.

    Article  Google Scholar 

  34. Mulvani, P. (1996). Surface plasmon spectroscopy of nanosized metal particles. Langmuir, 12, 788–800.

    Article  Google Scholar 

  35. Bellamy, L. J. (1975). The infra-red spectra of complex molecules (Vol. 1, 3rd ed., pp. 433). Halsted Press, a division of John Wiley & Sons, Inc., New York.

  36. Thompson, J. (1948). The correlation of vibrational absorption spectra with molecular structure. Journal of the Chemical Society, 328. https://doi.org/10.1039/JR9480000328.

  37. Ahmad, A., Mukherjee, P., Senapat, S., Mandal, D., Khan, M. I., Kumar, R., & Sastry, M. (2003). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and Surfaces. B, Biointerfaces, 28, 313–318.

    Article  Google Scholar 

  38. Ahmad, T., Wani, I. A., Manzoor, N., Ahmed, J., & Asiri, A. M. (2013). Biosynthesis, structural characterization and antimicrobial activity of gold and silver nanoparticles. Colloids and Surfaces. B, Biointerfaces, 107, 227–234.

    Article  Google Scholar 

  39. Viayakumar, M., Priya, K., Nancy, F. T., Noorlidah, A., & Ahmad, A. B. A. (2013). Biosynthesis, characterization and antibacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Industrial Crops and Products, 41, 235–240.

    Article  Google Scholar 

  40. Prabhu, S., & Poulose, E. K. (2012). Silver nanoparticles: Mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters, 2, 32.

    Article  Google Scholar 

  41. Bharathi, D., Diviya Josebin, M., Vasantharaj, S., & Bhuvaneshwari, V. (2018). Biosynthesis of silver nanoparticles using stem bark extracts of Diospyros montana and their antioxidant and antibacterial activities. Journal of Nanostructure in Chemistry, 8, 83–92.

    Article  Google Scholar 

  42. Hong, X., Wen, J., Xiong, X., & Hu, Y. (2016). Shape effect on the antibacterial activity of silver nanoparticles synthesized via a microwave-assisted method. Environmental Science and Pollution Research, 23, 4489–4497.

    Article  Google Scholar 

  43. Morones, J. R., Elechiguerra, J. L., Camacho, A., Holt, K., Kouri, J. B., Ramırez, J. T., & Yacaman, M. J. (2005). The bactericidal effect of silver nanoparticles. Nanotechnology, 16, 2346–2353.

    Article  Google Scholar 

  44. Kvitek, L., Panacek, A., Soukupova, J., Kolar, M., Vecerova, R., Prucek, R., Holecova, M., & Zboril, R. (2008). Effect of surfactants and polymers on stability and antibacterial activity of silver nanoparticles (NPs). Journal of Physical Chemistry C, 112, 5825–5834.

    Article  Google Scholar 

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Acknowledgments

We acknowledge with thanks the assistance provided by the University Sophisticated Instrument Facility (USIF) and the Department of Microbiology, Jawaharlal Nehru Medical College & Hospital, Aligarh Muslim University, Aligarh, India.

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Authors and Affiliations

  1. Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India

    Khwaja Salahuddin Siddiqi

  2. Department of Saidla, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India

    M. Rashid &  Tajuddin

  3. Department of Biology, College of Natural and Computational Sciences, University of Gondar, P.O. Box #196, Gondar, Ethiopia

    Azamal Husen

  4. Department of Ilmul Advia, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India

    Sumbul Rehman

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  1. Khwaja Salahuddin Siddiqi
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Siddiqi, K.S., Rashid, M., Tajuddin et al. Biofabrication of Silver Nanoparticles from Diospyros montana, Their Characterization and Activity Against Some Clinical Isolates. BioNanoSci. 9, 302–312 (2019). https://doi.org/10.1007/s12668-019-00629-9

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