Skip to main content

Advertisement

SpringerLink
Log in

Development of 36 novel polymorphic microsatellites for the critically endangered tree Pinus squamaia, by transcriptome database mining

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Pinus squamaia X. W. Li is a critically endangered five-needle pine species, with only 35 wild individuals left. To understand its genetic variability, 506 novel microsatellite markers were developed by mining the transcriptome database of P. lambertiana. 196 loci were able to amplify a fragment of expected size in P. squamaia, of which 36 were polymorphic. The number of alleles of these 36 EST-SSRs in 16 individuals varied from 2 to 4, with an average of 2.2. The observed (Ho) and expected (He) heterozygosity values ranged from 0.000 to 1.000 (averaging 0.564) and 0.061 to 0.500 (averaging 0.356), respectively. Twenty-four loci showed significant departure from HWE after Bonferroni correction. This was the first report of candidate molecular markers on P. squamaia, which will facilitate future analyses of genetic diversity and population structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Kalinowski ST, Taper ML, Marshall TC (2007) Riving how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106

    Article  PubMed  Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28:2537–2539

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Zhang ZY, Li DZ (2003) Conservation genetics of an extremely endangered pine, Pinus squamata. Acta Bot Yunnanica 25(5):544–550

    CAS  Google Scholar 

Download references

Acknowledgments

We thank Wen-hu Li for helps in sample collection. This work was financially supported by grants from the “Beijing Higher Education Young Elite Teacher Project (No. YETP0756)”and the “Fundamental Research Funds for the Central Universities (No. YX2013-41)”.

Author information

Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Qinghua East Road No. 35, Beijing, 100083, People’s Republic of China

    Jian-Feng Mao, Hui Liu & Ying-Yue Li

  2. Department of Biology and Chemistry, Baoding University, Baoding, 071000, Hebei, People’s Republic of China

    Bin-Bin Cui

  3. National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Zhi-Yong Zhang

Authors
  1. Jian-Feng Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bin-Bin Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhi-Yong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ying-Yue Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ying-Yue Li.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 115 kb)

Supplementary material 2 (PDF 331 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mao, JF., Cui, BB., Zhang, ZY. et al. Development of 36 novel polymorphic microsatellites for the critically endangered tree Pinus squamaia, by transcriptome database mining. Conservation Genet Resour 7, 93–94 (2015). https://doi.org/10.1007/s12686-014-0300-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0300-y

Keywords

Search

Navigation