Abstract
The coastal pelagic fish Normanichthys crockeri inhabits the southeast Pacific coast and is a monotypic species of the Normanichthyidae family. Its ecologically and economic importance, and the high fishing pressure it is subjected to make N. crockeri an ideal target species for conservation. Here, we present 12 new hypervariable loci for this species. Allelic richness varied between 2 and 8 alleles, and observed heterozygosity ranged between 0.059 and 1.00. Two loci showed significant deviations from Hardy–Weinberg and revealed the presence of null alleles. These new loci have a high potential to be used in population genetics studies and fishery management plans.
References
Chevreux B, Wetter T, Suhai S (1999) Genome sequence assembly using trace signals and additional sequence information. Comput Sci Biol 99:45–56
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Res 8:92–94
Gómez A, Caballero L, Aranis A, Ramírez M (2012) Seguimiento general de pesquerías de peces y crustáceos. Pesquería pelágica Zona Centro-Sur 2012. SUBPESCA. Instituto de Fomento Pesquero
Huang XQ, Madan A (1999) CAP3: a DNA sequence assembly program. Genome Res 9:868–877
Vega E, Pequeño G (1993) Contribución al conocimiento biológico de Normanichthys crockeri Clark, 1937 (Osteichthyes, Scorpaeniformes). Rev Biol Mar 28:1–36
Acknowledgments
We thank to Sandra Ferrada and Santiago Miranda for their assistance on this work. This study was funded by FONDECYT Grants # 1130868 to AB and # 11100334 to EH-M. EH-M received additional funding from INCAR (FONDAP Project No. 15110027).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Silva, F., Hernández-Miranda, E. & Brante, A. New polymorphic microsatellite markers for the pelagic fish Normanichthys crockeri . Conservation Genet Resour 7, 493–495 (2015). https://doi.org/10.1007/s12686-014-0404-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0404-4