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Analysis of Saccharina japonica transcriptome using the high-throughput DNA sequencing technique and its vanadium-dependent haloperoxidase gene

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Abstract

Saccharina is one of the most important cold-water living marine brown algal genera. In this study we analyzed the transcriptome of S. japonica, which belongs to the 1 000 Plants (OneKP) Project, by using a next-generation high-throughput DNA sequencing technique. About 5.16 GB of raw data were generated, and 65 536 scaffolds with an average length of 454 bp were assembled with SOAP de novo assembly method. In total, 19 040 unigenes were identified by BLAST; 25 734 scaffolds were clustered into 37 Gene ontology functional groups; 6 760 scaffolds were classified into 25 COG categories, as well as 2 665 scaffolds that were assigned to 306 KEGG pathways. Majority of the unigenes exhibited more similarities to algae including brown algae and diatom than other cyanobacteria, marine diatom, and plant. Saccharina japonica has the outstanding capability to accumulate halogen such as Br and I via halogenation processes from seawater. We acquired 42 different vanadium-dependent haloperoxidases (vHPO) in S. japonica transcriptome data, including 5 segments of vanadium-dependent iodoperoxidase (vIPO) and 37 segments of vanadium-dependent bromoperoxidase (vBPO). Complicated analyses of identified fulllength S. japonica vBPO1 and S. japonica vBPO2 revealed the importance of vBPO among species of brown algae and the strong relationship between marine algal vBPOs and vIPOs. This study will enhance our understanding of the biological characteristics and economic values of S. japonica species.

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, Qingdao, 266003, China

    Xiayuan Liang, Shan Chi, Cui Liu & Tao Liu

  2. CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China

    Xumin Wang, Shuangxiu Wu, Jing Sun & Jun Yu

  3. Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China

    Xumin Wang, Shuangxiu Wu, Jing Sun & Jun Yu

  4. Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism, Zhong Shan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China

    Shengping Chen

Authors
  1. Xiayuan Liang
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  2. Xumin Wang
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  3. Shan Chi
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  4. Shuangxiu Wu
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  5. Jing Sun
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  6. Cui Liu
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  7. Shengping Chen
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  8. Jun Yu
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  9. Tao Liu
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Corresponding authors

Correspondence to Xumin Wang or Tao Liu.

Additional information

Foundation item: The National Natural Science Foundation of China under contract Nos 41206116, 31140070 and 31271397; Technology Project of Ocean and Fisheries of Guangdong Province under contract No. A201201E03; the Fundamental Research Funds for the Central Universities under contract No. 201262003; the algal transcriptome sequencing was supported by OneKP Project (www.onekp.com).

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Liang, X., Wang, X., Chi, S. et al. Analysis of Saccharina japonica transcriptome using the high-throughput DNA sequencing technique and its vanadium-dependent haloperoxidase gene. Acta Oceanol. Sin. 33, 27–36 (2014). https://doi.org/10.1007/s13131-014-0438-1

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  • DOI: https://doi.org/10.1007/s13131-014-0438-1

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