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Study of polyethylene glycol-fluorophore complex formation by fluorescence correlation spectroscopy

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Abstract

Fluorescent labelling and detection has become a powerful tool in many life sciences applications. Fluorescently-tagged molecules are reliably demarcated from their surroundings allowing for the interrogation of various structural or dynamic molecular or system properties. For reliable data acquisition or to harvest the potential of noncovalent labelling, it is important that there is a good understanding of potential interactions between the fluorophore and the environment. Here, for the first time we report a complexation between Atto fluorophores and a polyethylene glycol (PEG) polymer. Atto fluorophores have long life times, high quantum yield and high sensitivity and are thus utilized in applications such as live cell imaging, spectroscopy and super resolution microscopy. PEG, on the other hand, is one of the most widely utilized polymers in many biomedical fields such as drug delivery and tissue engineering. Here, we used fluorescence correlation spectroscopy to study the specificity and the mechanism of the complexation. We determined that it was specific to the fluorophore-polymer-solvent system studied and that it was caused by a hydrogen bonding between the carboxyl group of the Atto fluorophores and the ether oxygen of PEG.

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Authors and Affiliations

  1. Biomedical Engineering Department, Saint Louis University, St. Louis, MO, USA

    Yasaman Chehreghanianzabi & Silviya Petrova Zustiak

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  1. Yasaman Chehreghanianzabi
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  2. Silviya Petrova Zustiak
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Correspondence to Silviya Petrova Zustiak.

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Chehreghanianzabi, Y., Zustiak, S.P. Study of polyethylene glycol-fluorophore complex formation by fluorescence correlation spectroscopy. Macromol. Res. 24, 995–1002 (2016). https://doi.org/10.1007/s13233-016-4142-1

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  • DOI: https://doi.org/10.1007/s13233-016-4142-1

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