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Genetic diversity, population structure, and association mapping of biomass traits in maize with simple sequence repeat markers

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Abstract

Assessing genetic diversity, population structure, and linkage disequilibrium is important in identifying potential parental lines for breeding programs. In this study, we assessed the genetic and phenotypic variation of 174 normal maize (Zea mays) inbred lines and made association analyses with respect to nine agronomical traits, using 150 simple sequence repeats (SSR). From population structure analysis, the lines were divided into three groups. Association analysis was done with a mixed linear model and a general linear model. Twenty-one marker-trait associations involving 19 SSR markers were observed using the mixed model, with a significance level of P < 0.01. All of these associations, as well as 120 additional marker-trait associations involving 77 SSR markers, were observed with the general model. Two significant marker-trait associations (SMTAs) were detected at P ≤ 0.0001. In the mixed linear model, one locus was associated with water content, two loci were associated with 100-kernel weight, setted ear length, ear thickness and stem thickness; three loci were associated with ear height, four loci were associated with total kernel weight and five loci were associated with plant height. These results should prove useful to breeders in the selection of parental lines and markers.

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Acknowledgments

This study was supported by a Korea Research Foundation Grant funded by the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center, No. PJ0080182014) of the Rural Development Administration, Republic of Korea, and Golden Seed Project (No. 213001-04-1-SBA10), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development of Korea (RDA), and Korea Forest Service (KFS).

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    Authors and Affiliations

    1. Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 200-701, Korea

      Jong Yeol Park, Rahul Vasudeo Ramekar, Kyu Jin Sa & Ju Kyong Lee

    2. Maize Experiment Station, Gangwon Agricultural Research and Extension Services, Hongcheon, 250-823, South Korea

      Jong Yeol Park

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    1. Jong Yeol Park
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    2. Rahul Vasudeo Ramekar
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    Correspondence to Ju Kyong Lee.

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    Jong Yeol Park, R. V. Ramekar, and K. J. Sa contributed equally to this work.

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    Park, J.Y., Ramekar, R.V., Sa, K.J. et al. Genetic diversity, population structure, and association mapping of biomass traits in maize with simple sequence repeat markers. Genes Genom 37, 725–735 (2015). https://doi.org/10.1007/s13258-015-0309-y

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