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Clonal structure of Ceratocystis manginecans populations from mango wilt disease in Oman and Pakistan

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Abstract

Ceratocystis manginecans has recently been described from Oman and Pakistan where the fungus causes a serious wilt disease of mango. In both countries, the disease has moved rapidly throughout mango producing areas leading to the mortality of thousands of mango trees. The disease is associated with the infestation of the wood-boring beetle Hypocryphalus mangiferae that consistently carries C. manginecans. The aim of this study was to consider the population structure of C. manginecans isolated from Oman and Pakistan using microsatellite markers and amplified fragment length polymorphisms (AFLPs). Population genetic analysis of C. manginecans isolates from diseased mango tissue and bark beetles associated with the disease in Oman and Pakistan, showed no genetic diversity. The apparently clonal nature of the population suggests strongly that C. manginecans was introduced into these countries as a single event or from another clonal source.

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Acknowledgment

We thank the members of the Tree Protection Co-operative Programme (TPCP), National Research Foundation (NRF), South Africa and the Ministry of Agriculture and Fisheries in Sultanate of Oman for funding this research. The Nuclear Institute for Agriculture and Biology (NIAB), Pakistan, is acknowledged for facilitating the collection of isolates. We also thank Lieschen de Vos for assistance with the AFLP experiments and William M. Deadman for assistance with map design.

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Authors and Affiliations

  1. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa

    A. O. Al Adawi, I. Barnes, B. D. Wingfield & M. J. Wingfield

  2. Ghadafan Agriculture Research Station, Ministry of Agriculture and Fisheries, P. O. Box 204, Sohar, 311, Sultanate of Oman

    A. O. Al Adawi

  3. University of Agriculture, Faisalabad, 38040, Pakistan

    I. A. Khan

  4. Department of Crop Sciences, Sultan Qaboos University, P.O. Box 34, Al Khod, 123, Sultanate of Oman

    M. L. Deadman

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  1. A. O. Al Adawi
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  2. I. Barnes
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Correspondence to I. Barnes.

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Fig S1

Polyacrylamide gel showing AFLP fragments amplified with Mo1 + ECC primer combination. Lanes 1, 24: molecular weight marker (range of 50-700 bp), Lanes 2 -11: Ceratocystis manginecans isolates from Oman isolated from Mangifera indica (lanes 2-6) and Hypocryphalus mangiferae (lanes 7-11). Lanes 12-23: C. manginecans isolates from Pakistan isolated from M. indica (lanes 12-17) and H. mangiferae (lanes 18-23). Lanes 25-27: C. platani from Platanus, used as controls. (PDF 295 kb)

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Al Adawi, A.O., Barnes, I., Khan, I.A. et al. Clonal structure of Ceratocystis manginecans populations from mango wilt disease in Oman and Pakistan. Australasian Plant Pathol. 43, 393–402 (2014). https://doi.org/10.1007/s13313-014-0280-0

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