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Preparation and Characterization of Nanocomposite Scaffolds (Collagen/β-TCP/SrO) for Bone Tissue Engineering

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Nowadays, production of nanocomposite scaffolds based on natural biopolymer, bioceramic, and metal ions is a growing field of research due to the potential for bone tissue engineering applications.

Methods:

In this study, a nanocomposite scaffold for bone tissue engineering was successfully prepared using collagen (COL), beta-tricalcium phosphate (β-TCP) and strontium oxide (SrO). A composition of β-TCP (4.9 g) was prepared by doping with SrO (0.05 g). Biocompatible porous nanocomposite scaffolds were prepared by freeze-drying in different formulations [COL, COL/β-TCP (1:2 w/w), and COL/β-TCP-Sr (1:2 w/w)] to be used as a provisional matrix or scaffold for bone tissue engineering. The nanoparticles were characterized by X-ray diffraction, Fourier transforms infrared spectroscopy and energy dispersive spectroscopy. Moreover, the prepared scaffolds were characterized by physicochemical properties, such as porosity, swelling ratio, biodegradation, mechanical properties, and biomineralization.

Results:

All the scaffolds had a microporous structure with high porosity (~ 95–99%) and appropriate pore size (100–200 μm). COL/β-TCP-Sr scaffolds had the compressive modulus (213.44 ± 0.47 kPa) higher than that of COL/β-TCP (33.14 ± 1.77 kPa). In vitro cytocompatibility, cell attachment and alkaline phosphatase (ALP) activity studies performed using rat bone marrow mesenchymal stem cells. Addition of β-TCP-Sr to collagen scaffolds increased ALP activity by 1.33–1.79 and 2.92–4.57 folds after 7 and 14 days of culture, respectively.

Conclusion:

In summary, it was found that the incorporation of Sr into the collagen-β-TCP scaffolds has a great potential for bone tissue engineering applications.

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Acknowledgement

We thank Tarbiat Modares University for supporting this work.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Jalal ale Ahmad Highway, P.O. Box 14115-114, Tehran, Iran

    Hamid Goodarzi & Sameereh Hashemi-Najafabadi

  2. Tissue Engineering and Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Jalal ale Ahmad Highway, P.O. Box 14115-331, Tehran, Iran

    Nafiseh Baheiraei

  3. Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Jalal ale Ahmad Highway, P.O. Box 14115-114, Tehran, Iran

    Fatemeh Bagheri

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  1. Hamid Goodarzi
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Correspondence to Sameereh Hashemi-Najafabadi or Nafiseh Baheiraei.

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Animal experiments were approved by the Ethics Committee of Tarbiat Modares University, Iran (IR.TMU.REC.1395.412).

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Goodarzi, H., Hashemi-Najafabadi, S., Baheiraei, N. et al. Preparation and Characterization of Nanocomposite Scaffolds (Collagen/β-TCP/SrO) for Bone Tissue Engineering. Tissue Eng Regen Med 16, 237–251 (2019). https://doi.org/10.1007/s13770-019-00184-0

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