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Human Fetal Cartilage-Derived Progenitor Cells Exhibit Anti-Inflammatory Effect on IL-1β-Mediated Osteoarthritis Phenotypes In Vitro

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

In this study, we have investigated whether human fetal cartilage progenitor cells (hFCPCs) have anti-inflammatory activity and can alleviate osteoarthritis (OA) phenotypes in vitro.

Methods:

hFCPCs were stimulated with various cytokines and their combinations and expression of paracrine factors was examined to find an optimal priming factor. Human chondrocytes or SW982 synoviocytes were treated with interleukin-1β (IL-1β) to produce OA phenotype, and co-cultured with polyinosinic-polycytidylic acid (poly(I-C))-primed hFCPCs to address their anti-inflammatory effect by measuring the expression of OA-related genes. The effect of poly(I-C) on the surface marker expression and differentiation of hFCPCs into 3 mesodermal lineages was also examined.

Results:

Among the priming factors tested, poly(I-C) (1 µg/mL) most significantly induced the expression of paracrine factors such as indoleamine 2,3-dioxygenase, histocompatibility antigen, class I, G, tumor necrosis factor- stimulated gene-6, leukemia inhibitory factor, transforming growth factor-β1 and hepatocyte growth factor from hFCPCs. In the OA model in vitro, co-treatment of poly(I-C)-primed hFCPCs significantly alleviated IL-1β-induced expression of inflammatory factors such as IL-6, monocyte chemoattractant protein-1 and IL-1β, and matrix metalloproteinases in SW982, while it increased the expression of cartilage extracellular matrix such as aggrecan and collagen type II in human chondrocytes. We also found that treatment of poly(I-C) did not cause significant changes in the surface marker profile of hFCPCs, while showed some changes in the 3 lineages differentiation.

Conclusion:

These results suggest that poly(I-C)-primed hFCPCs have an ability to modulate inflammatory response and OA phenotypes in vitro and encourage further studies to apply them in animal models of OA in the future.

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Acknowledgements

This research was supported by a grant of the Korea Health Technology R&D Project funded by the Ministry of Health & Welfare, Republic of Korea (HI17C2191) and the National Research Foundation Grant (NRF-2019M3E5D1A02070861).

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Inha University College of Medicine, Incheon, 22212, Korea

    Jiyoung Kim & So Ra Park

  2. Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Korea

    An Nguyen-Thuy Tran & Byoung-Hyun Min

  3. Cell Therapy Center, Ajou University Medical Center, Suwon, 16499, Korea

    An Nguyen-Thuy Tran & Byoung-Hyun Min

  4. Department of Biomedical Sciences, Inha University College of Medicine, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Korea

    Ji Young Lee & Byung Hyune Choi

  5. Department of Biomedical Engineering, Pukyong National University, Pusan, 48513, Korea

    Sang-Hyug Park

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  1. Jiyoung Kim
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Correspondence to Byung Hyune Choi.

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The experiments were conducted with the approval of institutional review board (IRB) of Ajou University Medical Center (AJIRB-CRO-07-139) and (AJIRB-MED-SMP-10–266).

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Kim, J., Tran, A.NT., Lee, J.Y. et al. Human Fetal Cartilage-Derived Progenitor Cells Exhibit Anti-Inflammatory Effect on IL-1β-Mediated Osteoarthritis Phenotypes In Vitro. Tissue Eng Regen Med 19, 1237–1250 (2022). https://doi.org/10.1007/s13770-022-00478-w

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