Abstract
Pseudomonas fluorescens isolates were screened from the rhizosphere soil of tobacco-growing fields of Andhra Pradesh and Karnataka. Antagonistic activity of isolates was observed against Rhizoctonia solani. In order to obtain a fluorescent Pseudomonas strain having capacity to reduce the disease symptoms produced by R. solani, six P. fluorescens strains with moderate and high fungal growth inhibition capacity were tested in vitro. Tobacco seed beds pre-inoculated with R. solani at 108 cfu ml−1 concentration were treated with six promising isolates of P. fluorescens obtained from tobacco rhizosphere. Despite the differences found in the dynamics of colonization and colonization capacity, all evaluated strains induced tobacco growth and reduced disease symptoms produced by R. solani. Present investigation clearly indicates that establishment of P. fluorescens strains in tobacco rhizosphere is a feasible alternative for the management of R. solani symptoms. P. fluorescens when applied as a biocontrol agent on tobacco seed beds showed appreciable increase in the biometric parameters of tobacco seedlings.
Similar content being viewed by others
References
Akram S, Reza AH, Askari H, Naderi DQ, Farsi M, Eslamd MH (2009) Biocontrol of Rhizoctonia solani damping-off of sugar beet with native Streptomyces strains under field conditions. Biocontrol Sci Tech 19(9):985–991
Amel AH, Soad MA, Ahmed I (2010) Activation of tomato plant defence response against Fusarium wilt disease using Trichoderma harzianum and salicylic acid under greenhouse conditions. Res J Agric Biol Sci 6:328–338
Askar AA, Rasnae YA (2010) Arbuscular mycorrhizal fungi: a biocontrol agent’s against common bean Fusarium root rot disease. Plant Pathol J 9:31–38
Becker J, Eisenhauer N, Scheu S, Jousset A (2012) Increasing antagonistic interactions cause bacterial communities to collapse at high diversity. Ecol Lett 15(5):468–474
Carruthers FL, Conner AJ, Mahanty HK (1994) Identification of a genetic locus in P. aureofaciens involved in fungal inhibition. Appl Environ Microbiol 60:71–77
Chet I (1990) Biological control of soilborne pathogens with fungal antagonists in combination with soil treatments. In: Hornby D, Cook RJ, Henis Y, Ko WH, Rovira AD, Schippers B, Scott PR (eds) Biological control of soilborne pathogens. CAB Publishing House, NY, pp 15–25
De Freitas JR, Germida JJ (1991) Pseudomonas cepacia and Pseudomonas putida as winter wheat inoculants for biocontrol of Rhizoctonia solani. Can J Microbiol 37:780–784
Gamalero E, Fracchia L, Cavaletto M, Garvaye J, Frey-Klett P, Varese GC, Martinotti MG (2003) Characterization of functional traits of two fluorescent pseudomonads isolated from basidiomes of ectomycorrhizal fungi. Soil Biol Biochem 35:55–65
Ganesan S, Sekar R (2004) Biocontrol mechanism of Trichoderma harzianum (ITCC-4572) on groundnut web blight disease caused by Rhizoctonia solani. J Theor Exp Biol 1:43–47
Guleria S, Aggarwal R, Thind TS, Sharma TR (2007) Morphological and pathological variability in rice isolates of Rhizoctonia solani and molecular analysis of their genetic variability. J Phytopathol 155:654–661
Kloepper JW, Schroth MN, Miller TD (1980) Effects of rhizosphere colonization by plant growth promotion rhizobacteria on potato plant development and yield. Phytopathol 70:1078–1082
Mazzola M (1998) The potential of natural and genetically engineered fluorescent Pseudomonas spp. as bio control agents. In: Subba Rao NS, Dommergues YR (eds) Microbial interactions in agriculture and forestry, vol 1. Science publishers Inc, Enfield, pp 193–217
Nandakumar R, Babu S, Viswanathan R, Raguchander T, Samiyappan R (2001) Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biol Biochem 23:603–612
Nautiyal CS (1997) Rhizosphere competence of Pseudomonas sp. NBRI9926 and Rhizobium sp. NBRI9513 involved in the suppression of chickpea (Cicer arietinum L.) pathogenic fungi. FEMS Microbiol Ecol 23:145–158
Neha D, Dawande AY (2010) Biocontrol of soil borne plant pathogen Rhizoctonia solani using Trichoderma sp. and Pseudomonas fluorescens. Asiatic J Biotech Res 01:39–44
Nielsen TH, Sorensen D, Tobiasen C, Andersen JB, Christophersen C, Givskov M, Sorensen J (2002) Antibiotic and biosurfactant properties of cyclic lipopeptides produced by fluorescent Pseudomonas spp. from the sugar beet rhizosphere. Appl Environ Microbiol 68:3416–3423
Pal KK, Gardener BMS (2006) Biological control of plant pathogens. Plant Health Instr. doi:10.1094/PHI-A-2006-1117-02
Pandya U, Saraf M (2010) Application of fungi as a biocontrol agent and their biofertilizer potential in agriculture. J Adv Dev Res 1:90–99
Parke JT, Rand RE, Joy AE, King FB (1991) Biological control of Pythium damping-off and Aphanomyces root rot of peas by application of Pseudomonas cepacia or Pseudomonas fluorescens to seed. Plant Dis 75:987–992
Rangaswamy G (1958) Diseases of crop plants in India. Prentice hall of India Pvt.Ltd, New Delhi, p 504
Sindhu SS, Suneja S, Goel AK, Parmar N, Dadarwal KR (2002) Plant growth promoting effects of Pseudomonas sp. on coinoculation with Mesorhizobium sp. Cicer strain under sterile and “wilt sick” soil conditions. Appl Soil Ecol 19:57–64
Sneh B, Burpee L, Ogoshi A (1991) Identification of Rhizoctonia species. Academic, New York
Subhashini DV (2011) Studies on the beneficial bacteria obtained from tobacco rhizosphere. Indian Phytopathol 64:300–302
Subhashini DV (2012) Studies on efficacy of different delivery systems of Pseudomonas fluorescens for biosuppression of damping-off disease in tobacco seed beds. J Biol Control 26:84–87
Subhashini DV (2013) Effect of bio-inoculation of AM fungi and PGPR on the growth, yield and quality of FCV tobacco (Nicotiana tabacum) in vertisols. Indian J Agric Sci 83:667–672
Subhashini DV (2014) Genotype dependent variation in native and inoculated soil microorganisms of FCV tobacco (Nicotiana tabacum) rhizosphere in vertisols and alfisols. Indian J Agric Sci 84:272–275
Subhashini DV, Padmaja K (2009) Exploitation of Pseudomonas fluorescens for the management of damping-off disease of tobacco in seedbeds. Indian J Plant Prot 37:147–150
Subhashini DV, Padmaja K (2010) Effect of bioinoculants on seedling vigour in tobacco (Nicotiana tabacum) nurseries. Indian J Agric Sci 80:186–188
Subhashini DV, Padmaja K (2011) Potential of phosphate solubilising Pseudomonas as biofungicide. Arch Phytopathol Plant Prot 44:1041–1045
Suslow TV, Schroth MN (1982) Effects of seed application of rhizobacteria on root colonization yield of Sugarbeets fungal and bacterial phytopathogens. Phytopathology 72:199–206
Van Peer R, Punte HL, Weger LA, Schippers B (1990) Characterization of root surface and endorhizosphere pseudomonads in relation to their colonization of roots. Appl Environ Microbiol 56:2462–2470
Vidhyasekaran P, Muthamilan M (1995) Development of formulations of Pseudomonas fluorescens for the control of chickpea wilt. Plant Dis 79:782–786
Weller DM (1988) Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407
Weller DM (2007) Pseudomonas biocontrol agents of soilborne pathogens: looking back over 30 years. Phytopathol 97:250–256
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dandamudi Vallabhaneni, S. Biocontrol of Rhizoctonia solani in Tobacco (Nicotiana tabacum) Seed Beds Using Pseudomonas fluorescens . Agric Res 5, 137–144 (2016). https://doi.org/10.1007/s40003-016-0207-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40003-016-0207-9