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In vitro regeneration of Citrus hystrix DC

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Abstract

An efficient plantlet regeneration system for Citrus hystrix DC has been developed in this study. N 6-benzyladenine (BA) was most commonly used plant growth regulator in micropropagation of Citrus spp. However, our study demonstrated that BA alone was insufficient to induce normal in vitro shoot regeneration of C. hystrix due to the occurrence of leaf senescence on the regenerated shoots. The inclusion of silver nitrate (AgNO3) at 10.0 µM into Murashige and Skoog (MS) medium containing 2.22 µM BA could effectively overcome the leaf senescence and improved shoot regeneration from different explants (cotyledonary node, cotyledon, hypocotyl and primary root) of C. hystrix. In the rooting experiment, the application of continuous indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) treatment of 4.92, 9.84, 14.76, and 19.69 µM IBA or 1.34, 2.69, and 5.37 µM NAA in combination with 20.0 µM AgNO3 resulted in poor rooting of C. hystrix shoots. Rooting was significantly improved upon exposure of shoots to IBA pulse treatment of 9,840 µM for 16 h prior to their transfer on MS medium supplemented with 20.0 µM AgNO3. Assessment of various potting mixtures for acclimatization showed medium containing loamy soil: Peatgro: sand (1:1:1; v/v/v) produced high percentage (83.3 %) of plantlet survival.

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Acknowledgments

We wish to express our gratitude to the Department of Agrotechnology, Faculty of Agriculture, Universiti Putra Malaysia for providing the laboratory facilities and materials for this research.

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Authors and Affiliations

  1. Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Wee Hiang Eng & Maheran Abd Aziz

  2. Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Maheran Abd Aziz

  3. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Uma Rani Sinniah

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  1. Wee Hiang Eng
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  2. Maheran Abd Aziz
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  3. Uma Rani Sinniah
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Correspondence to Maheran Abd Aziz.

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Eng, W.H., Aziz, M.A. & Sinniah, U.R. In vitro regeneration of Citrus hystrix DC. Braz. J. Bot 38, 235–242 (2015). https://doi.org/10.1007/s40415-014-0127-5

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  • DOI: https://doi.org/10.1007/s40415-014-0127-5

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