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Pre-colonized seedlings with arbuscular mycorrhizal fungi: an alternative for the cultivation of Jatropha curcas L. in salinized soils

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Abstract

The aim of this study was to test the hypothesis that Jatropha curcas L. seedlings previously inoculated with arbuscular mycorrhizal fungi (AMF) in non-salinized soil and later transplanted to salinized soil are more tolerant to saline stress. The experiment was conducted in greenhouse using a randomized block design in a 4 × 2 factorial scheme. The first factor consisted of salinized soil with electrical conductivity (EC) of 2, 5, 8 and 10 dS/m, and the second consisted of non-colonized plants, and plants that were pre-colonized with AMF, with eight replicates. Salt had a deleterious effect on plants non-inoculated with AMF, which showed reduced growth and photosynthetic activity and increased stress indicators, such as lipid peroxidation, proline accumulation, and high antioxidant enzymatic activity. On the other hand, pre-colonized plants were less affected by the presence of salt in the soil. Seedlings pre-inoculated with AMF presented the same growth rate and photosynthetic activity in all saline treatments. No increase in lipid peroxidation and antioxidant enzymatic activity was observed in seedlings pre-inoculated with AMF even under high saline concentration of 10 dS/m EC. The results suggest the existence of a mechanism to mitigate the effects of saline stress on pre-colonized seedlings. Therefore, pre-colonized J. curcas seedlings are an alternative for the cultivation of this species in salinized soils.

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Acknowledgement

We are indebted to International Collection of Glomeromycota Culture (CICG, Blumenau, Brazil, www.furb.br/cicg), for providing the isolated cultures of arbuscular mycorrhizal fungi for this study.

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  1. Federal Institute of Education Science and Technology of Alagoas, Campus of Maceió, 530 Ferroviario Avenue, Downtown, Maceió, AL, 57020-600, Brazil

    Dilliani Felipe Barros de Oliveira

  2. Center of Agrarian Sciences, Federal University of Alagoas, Br 104 North, Km 85, Rio Largo, AL, 57072-900, Brazil

    Dilliani Felipe Barros de Oliveira, Lauricio Endres, José Vieira Silva & Paulo Ricardo Aprígio Clemente

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  1. Dilliani Felipe Barros de Oliveira
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  2. Lauricio Endres
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  3. José Vieira Silva
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Correspondence to Dilliani Felipe Barros de Oliveira.

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de Oliveira, D.F.B., Endres, L., Silva, J.V. et al. Pre-colonized seedlings with arbuscular mycorrhizal fungi: an alternative for the cultivation of Jatropha curcas L. in salinized soils. Theor. Exp. Plant Physiol. 29, 129–142 (2017). https://doi.org/10.1007/s40626-017-0089-7

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