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Tetraploid induction of Crassostrea hongkongensis and C. sikamea by inhibiting the polar body 1 release in diploid fertilized eggs

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Abstract

The production of an all-triploid population by mating tetraploid males with diploid females is the best and most fundamental method for the large-scale production of triploid oysters. Obtaining a stable tetraploid population is essential for guaranteed production in industrialized triploid cultivation. C. hongkongensis and C. sikamea are important oyster breeding species in southern China, and have great economic value. However, there are not any published data on inducing tetraploid C. hongkongensis or C. sikamea. Therefore, we investigated tetraploid induction in these two oyster species by inhibiting the PB1 release in diploid fertilized eggs using Cytochalasin B (CB) under 31 °C, 15 ‰ salinity. The results confirmed that the optimal tetraploid induction conditions for C. hongkongensis were a CB concentration of 0.50 mg/L with induction starting at 9.0 min after fertilization, and stopping at 21.0 min after fertilization; the induction efficiency index reached 0.123 under these conditions. The optimal tetraploid induction conditions for C. sikamea were a CB concentration of 0.50 mg/L, with induction starting at 7.5 min after fertilization and stopping at 18 min after fertilization; the induction efficiency index could be as high as 0.281 under these conditions. However, we confirmed that the tetraploid rate decreased with larval growth, and no tetraploids were detected in the juvenile period of either C. hongkongensis or C. sikamea. This may be attributed to the very low survival of the tetraploid larvae induced by this method, especially as most tetraploid larvae died during the first three days. In summary, it is simple to directly induce tetraploid C. hongkongensis and C. sikamea larvae by inhibiting the PB1 release of diploid zygotes, but the low survival rate makes it challenging to obtain viable juvenile tetraploids.

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Acknowledgements

This research was supported by the National Science Foundation of China (32002387); the Chinese Ministry of Science and Technology through the National Key Research and Development Program of China (2018YFC1406505; 2018YFD0901400; 2020YFD0901102); Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0404); the Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (ISEE2018PY01, ISEE2018ZD02, ISEE2018PY03); the China Agriculture Research System Project (CARS-49), and the Science and Technology Planning Project of Guangdong Province, China (2017B030314052).

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Authors and Affiliations

  1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Yanping Qin, Zohaib Noor, Xingyou Li, Haitao Ma, Jun Li, Yinyin Zhou, Yuehuan Zhang & Ziniu Yu

  2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 510301, China

    Yanping Qin, Zohaib Noor, Xingyou Li, Haitao Ma, Jun Li, Yinyin Zhou, Yuehuan Zhang & Ziniu Yu

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Zohaib Noor, Xingyou Li & Yinyin Zhou

  4. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, China

    Yanping Qin, Haitao Ma, Jun Li, Yuehuan Zhang & Ziniu Yu

  5. Guangxi A Bang-Ding Marine Technology Company, Nanning, 530000, China

    Riguan Mo

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  1. Yanping Qin
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  2. Zohaib Noor
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  5. Jun Li
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  7. Riguan Mo
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  8. Yuehuan Zhang
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  9. Ziniu Yu
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Contributions

YQ, ZY, and YZ designed experiments. YQ carried out all of the experiments with the help of ZN, XL, JL, HM, YZ and RM. YQ analyzed the data and wrote the paper. ZY and YZ critically revised the manuscript and approved the final version to be published.

Corresponding authors

Correspondence to Yuehuan Zhang or Ziniu Yu.

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The author declares that there is no conflict of interest.

Animal and human rights statement

This study was conducted in accordance with the Institutional Animal Care and Use Committee of South China Sea Institute of Oceanology, Chinese Academy of Sciences, and it does not contain any studies with human participants.

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Edited by Xin Yu.

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Qin, Y., Noor, Z., Li, X. et al. Tetraploid induction of Crassostrea hongkongensis and C. sikamea by inhibiting the polar body 1 release in diploid fertilized eggs. Mar Life Sci Technol 3, 463–473 (2021). https://doi.org/10.1007/s42995-021-00107-w

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  • DOI: https://doi.org/10.1007/s42995-021-00107-w

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