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Electric properties of thylakoid membranes from pea mutants with modified carotenoid and chlorophyll–protein complex composition

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Abstract

Surface electric properties of thylakoid membranes from wild type and two mutant forms, Coeruleovireus 2/16 and Costata 2/133, of pea are investigated by electric light scattering and microelectrophoretic measurements. Characterization of the chlorophyll–protein complexes in thylakoid membranes reveals that the relative ratio of oligomeric (LHC II1) to monomeric (LHC II3) forms of the light-harvesting Chl a/b complex of Photosystem II is lower (3.34) in 2/133 mutant and higher (6.62) in 2/16 mutant than in wild type (4.57). This is accompanied by elevated amounts and a considerable reduction of all carotenoids in 2/16 and 2/133 mutant, respectively, as compared to the wild type. The concomitant variations of the permanent dipole moment (transversal charge asymmetry), electric polarizability and electrokinetic charge of the thylakoid membranes from both the mutants are discussed in terms of the differences in the supramolecular (oligomeric) organization of the light-harvesting complexes II within the photosynthetic apparatus.

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Authors and Affiliations

  1. Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria

    Anelia Dobrikova, Alexander G. Ivanov & Emilia Apostolova

  2. Department of Plant Sciences, University of Western Ontario, London, Ontario, Canada, N6A 5B7

    Rachael M. Morgan, Alexander G. Ivanov & Norman P.A. Huner

  3. Institute of Physical Chemistry, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria

    Ivana Petkanchin

  4. Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria

    Stefka G. Taneva

Authors
  1. Anelia Dobrikova
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  2. Rachael M. Morgan
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  3. Alexander G. Ivanov
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  4. Emilia Apostolova
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  5. Ivana Petkanchin
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  6. Norman P.A. Huner
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  7. Stefka G. Taneva
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Correspondence to Stefka G. Taneva.

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Dobrikova, A., Morgan, R.M., Ivanov, A.G. et al. Electric properties of thylakoid membranes from pea mutants with modified carotenoid and chlorophyll–protein complex composition. Photosynthesis Research 65, 165–174 (2000). https://doi.org/10.1023/A:1006428631432

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  • DOI: https://doi.org/10.1023/A:1006428631432

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