Abstract
Banana streak virus (BSV), a member of the Badnavirus group of plant viruses, causes severe problems in banana cultivation, reducing fruit yield and restricting plant breeding and the movement of germplasm. Current detection methods are relatively insensitive. In order to develop a PCR-based diagnostic method that is both reliable and sensitive, the genome of a Nigerian isolate of BSV has been sequenced and shown to comprise 7389 bp and to be organized in a manner characteristic of badnaviruses. Comparison of this sequence with those of other badnaviruses showed that BSV is a distinct virus. PCR with primers based on sequence data indicated that BSV sequences are present in the banana genome.
Similar content being viewed by others
References
Lockhart B.E.L., Phytopathology 76, 995-999, 1986.
Lockhart B.E.L., Phytopathology 80, 127-131, 1990.
Medberry S.L., Lockhart B.E.L., and Olszewski N.E., Nucl Acid Res 18, 5505-5513, 1990.
Hay J.M., Jones M.C., Blakeborough M.L., Dasgupta I., Davis J.W., and Hull R., Nucl Acids Res 19, 2615-2621, 1991.
Qu R., Bhattacharyya M., Laco G.S., de Kochko A., Subba Rao B.L., Kaniewska M.B., Elmer J.S., Rochester D.E., Smith C.E., and Beachy R.N., Virology 185, 354-364, 1991.
Bouhida M., Lockhart B.E.L., and Olszewski N.E., J Gen Virol 74, 15-22, 1993.
Hagen L.S., Jacquemond M., Lepingle A., Lot H., and Tepfer M., Virology 196, 619-628, 1993.
Lockhart B.E.L. and Olszewski N.E., In: Breeding Banana and Plantain for Resistance to Diseases and Pests. J Ganry (ed.). CIRAD/INIBAP, Montpellier, France, pp 105-113, 1993.
Bejarano E.R., Khashoggi A., Witty M., and Lichtenstein C., Proc Natl Acad Sci 93, 759-764, 1996.
Richert-Pöggeler K.R. and Shepherd R.J., Virology 236, 137- 146, 1997.
LaFleur D.A., Lockhart B.E.L., and Olszewski N.E., Phytopathology 86, S100-S101, 1996.
Sambrook J., Fritsch E.F., and Maniatis T., Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York. 1989.
Devereux J., Haeberli P., and Smithies O., Nucl Acid Res 12, 387-395, 1984.
Li Q.B., Cai Q., and Guy C.L., Pl Molec Biol Rep 12, 215-220, 1994.
Feinberg A.P. and Vogelstein B., Anal Biochem 132, 6-13, 1983.
Fütterer J., Rothnie H.M., Hohn T., and Potrykus I., J Virol 71, 7984-7989, 1997.
Fütterer J., Potrykus I., Bao Y., Li L., Burns M., Hull R., and Hohn T., J Virol 70, 2999-3010, 1996.
Lutche H.A., Chow K.C., Mickel F.S., Moss K.A., Kern H.F., and Scheele G.A., EMBO J 6, 43-48, 1987.
Covey S.N., Nucl Acids Res 14, 623-633, 1986.
Hull R., Ann Rev Phytopathol 34, 275-297, 1996.
Lam E., Benfey P.N., Gilmartin P.M., Fang R.-X., and Chua N.H., Proc Natl Acad Sci USA 86:, 7890-7894, 1989.
Lam E. and Chua N.-H., Plant Cell 1, 147-1156, 1989.
Medberry S.L., Lockhart B.E.L., and Olszewski N.E., Plant Cell 4, 185-192, 1992.
Bao Y. and Hull R., Virology 197, 445-448, 1993.
Guilley H., Dudley R.K., Jonard G., Balàs E., and Richards K.E., Cell 30, 763-773, 1983.
Coffin J.M., Essex M., Gallo R., Graf T.M., Hinuma Y., Hunter E., Jaenisch R., Nusse R., Oroszlan S., Svoboda J., Teich N., Toyoshima K., and Varmus H., In Virus Taxonomy: Classification and nomenclature of viruses. (eds.) Murphy F.A., Fauquet C.M., Bishop D.H.L., Ghabrial S.A., Jarvis A.W., Martelli G.P., Mayo M.A., and Summers M.D. pp. 193-204, Springer Verlag, 1995.
Comstock J.C. and Lockhart B.E.L., Plant Disease 74, 530, 1990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harper, G., Hull, R. Cloning and Sequence Analysis of Banana Streak Virus DNA. Virus Genes 17, 271–278 (1998). https://doi.org/10.1023/A:1008021921849
Issue Date:
DOI: https://doi.org/10.1023/A:1008021921849