Abstract
A bacterium capable of degrading 2-methylpyridine was isolated by enrichment techniques from subsurface sediments collected from an aquifer located at an industrial site that had been contaminated with pyridine and pyridine derivatives. The isolate, identified as an Arthrobacter sp., was capable of utilizing 2-methylpyridine, 2-ethylpyridine, and 2-hydroxypyridine as primary C, N, and energy sources. The isolate was also able to utilize 2-, 3-, and 4-hydroxybenzoate, gentisic acid, protocatechuic acid and catechol, suggesting that it possesses a number of enzymatic pathways for the degradation of aromatic compounds. Degradation of 2-methylpyridine, 2-ethylpyridine, and 2-hydroxypyridine was accompanied by growth of the isolate and release of ammonium into the medium. Degradation of 2-methylpyridine was accompanied by overproduction of riboflavin. A soluble blue pigment was produced by the isolate during the degradation of 2-hydroxypyridine, and may be related to the diazadiphenoquinones reportedly produced by other Arthrobacter spp. when grown on 2-hydroxypyridine. When provided with 2-methylpyridine, 2-ethylpyridine, and 2-hydroxypyridine simultaneously, 2-hydroxypyridine was rapidly and preferentially degraded; however there was no apparent biodegradation of either 2-methylpyridine or 2-ethylpyridine until after a seven day lag. The data suggest that there are differences between the pathway for 2-hydroxypyridine degradation and the pathway(s) for 2-methylpyridine and 2-ethylpyridine.
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Aislabie J, Rothenburger S & Atlas RM (1989) Isolation of microorganisms capable of degrading isoquinoline under earobic conditions. Appl. Environ. Microbiol. 55: 3247–3249.
Bennett JL, Updegraff DM, Pereira WE & Rostad CE (1985) Isolation and identification of four species of quinoline-degrading pseudomonads from a creosote-contaminated site at Pensacola, Florida. Microbios Lett. 29: 147–154.
Blaschke M, Kretzer A, Schäfer C, Nagel M & Andreesen JR (1991) Molybdenum-dependent degradation of quinoline by Pseudomonas putida Chin IK and other aerobic bacteria. Arvh. Microbiol. 155: 164–169.
Bohonos J, Chow TW & Spanpggard RJ (1977) Some observations on biodegradation of pollutants in aquatic systems. Jap. J. Antibiot. 30: S275-S285.
Brockman FJ, Denovan BA, Hicks RJ & Fredrickson JK (1989) Isolation and characterization of quinoline-degrading bacteria from subsurface sediments. Appl. Environ. Microbiol. 55: 1029–1032.
Brown GM & Williamson JM (1982) Biosynthesis of riboflavin, folic acid, thiamin, and pantothenic acid. Adv. Enzymol. 53: 345–381.
Collins MD & Cummins CS (1986). Genus Corynebacterium. In: P. Sneath (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1266–1287). The Williams and Wilkins Co., Baltimore.
Cure GL & Keddie RM (1973) Methods for the morphological examination of aerobic coryneform bacteria. In: Board RG & Lovelock DN (Eds), Sampling — Microbiological Monitoring of Environments (pp. 123–135). Academic Press, New York.
Dobson KR, Stephenson M, Greenfield PF & Bell PRF (1985) Identification and treatability of organics in oil shale retort water. Wat. Res. 19: 849–856.
Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P (Ed), Manual of Methods for General Microbiology (p. 24). American Society for Microbiology, Washington, D.C.
Ensign JC & Rittenberg SC (1963) A crystalline pigment produced from 2-hydroxypyridine by Arthrobacter crystallopoietes n. sp. Archiv. Mikrobiol. 47: 137–153.
Ensign JC & Rittenberg SC (1964) The pathway of nicotinic acid oxidation by a Bacillus sp. J. Biol. Chem. 239: 2285–2291.
Feng Y, Kaiser J-P, Minard RD & Bollag J-M (1994) Microbial transformation of ethylpyridines. Biodegradation. 5: 121–128. Gherna RL, Richardson SH & Rittenberg SC (1965) J. Biol. Chem. 240: 3669–3674.
Godsy EM, Goerlitz DF & Grbic-Galic D (1992) Methanogenic biodegradation of creosote contaminants in natural and simulated ground-water ecosystems. Ground Water. 30: 232–242.
Golovlev EL (1976) Characteristics of the regulation of the microbial transformation of organic compounds. Chem. Abstr. 85: 156250x.
Goodfellow M (1986) Genus Rhodococcus. In: Williams S (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 4 (pp.2362–2371). The Williams and Wilkins Co., Baltimore.
Holmes PE & Rittenberg SC (1972) The bacterial oxidation of nicotine. VII. Partial purification and properties of 2,6-dihydroxypyridine oxidase. J. Biol. Chem. 247: 7622–7627.
Holmes PE, Rittenberg SC & Knackmuss HJ (1972) The bacterial oxidation of nicotine. VIII. Synthesis of 2,3,6-trihydroxypyridine and accumulation and partial characterization of the product of 2,6-dihydroxypyridine oxidation. J. Biol. Chem. 247: 7628–7633.
Houghton C & Cain RB (1972) Formation of pyridinediols (dihydroxypyridines) as intermediates in the degradation of pyridine compounds by micro-organisms. Biochem. J. 130: 879–893.
Jones D & Collins MD (1986) Irregular, nonsporing, Gram-positive rods. In: Sneath P (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1261–1266). The Williams and Wilkins Co., Baltimore.
Jones D & Keddie RM (1986) Genus Brevibacterium. In: Sneath P (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1301–1313). The Williams and Wilkins Co., Baltimore.
Keddie RM (1974) Genus Arthrobacter. In: Buchanan and Gibbons (Eds), Bergy's Manual of Determinative Bacteriology (pp. 618–625). 8 edn. The Williams and Wilkins Co., Baltimore.
Keddie RM, Collins MD & Jones D (1986) Genus Arthrobacter. In: Sneath, P. (Ed), Bergy's Manual of Determinative Bacteriology, Vol. 2 (pp. 1288–1301). The Williams and Wilkins Co., Baltimore.
Keeny DR & Nelson DW (1982) Nitrogen — Inorganic Forms. In: Page AL (Ed), Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties (pp. 674–676). Soil Science Society of America, Madison
Kloos WE, Tornabene TG & Schleifer KH (1974) Isolation and characterization of micrococci from human skin, including two new species: Micrococus lylae and Micrococcus kristinae. Int. J. Syst. Bacteriol. 24: 79–101.
Kocur M, Bergan T & Mortensen N (1971) DNA base composition of Gram-positive cocci. J. Gen. Microbiol. 69: 69.
Kolenbrander PE, Lotong N & Ensign JC (1976) Growth and pigment production by Arthrobacter pyridinolis n. sp. Arch. Microbiol. 110: 239–245.
Korosteleva LA, Kost AN, Vorob'eva LI, Modyanova LV, Terent'ev PB & Kulikov NS (1981) Microbiological degradation of pyridine and 3-methylpyridine. Appl. Biochem. Microbiol. 17: 276.
Kuhn EP & Suflita JM (1989) Microbial degradation of nitrogen, oxygen and sulfur heterocyclic compounds under anaerobic conditions: Studies with aquifer samples. Environ. Toxicol. Chem. 8: 1149–1158.
Kuhn R, Starr MP, Kuhn DA, Bauer H & Knackmuss HJ (1965) Indigoidine and other bacterial pigments related to 3,3′-bipyridyl. Arch. Mikrobiol. 51: 71–84.
Leenheer JA, Noyes TI & Stuber HA (1982) Determination of polar organic solutes in oil-shale retort water. Environ. Sci. Technol. 16: 714–723.
Leenheer JA & Stuber HA (1981) Migration through soil of organic solutes in an oil-shale process water. Environ. Sci. Technol. 15: 1467–1475.
Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
O'Loughlin EJ, Kehrmeyer SR & Sims GK (1995) Isolation, characterization and substrate utilization of a quinoline-degrading microorganism. Internat. Biodeterior. Biodegradat. 38: 107–118.
Pereira WE and Rostad CE (1985) Investigations of organic contaminants derived from wood-treatment processes in a sand and gravel aquifer near Pensacola, Florida. U.S. Geological Survey Water-Supply Paper 2290. U.S. Geological Survey, Denver, CO.
Pereira WE, Rostad CE, Garbarino JR & Hult MF (1983) Groundwater contamination by organic bases derived from coal-tar wastes. Environ. Toxicol. Chem. 2: 283–294.
Pereira WE, Rostad CE, Leiker TJ, Updegraff DM & Bennett JL. (1988) Microbial hydroxylation of quinoline in contaminated groundwater: Evidence for incorporation of the oxygen atom of water. Appl. Environ. Microbiol. 54: 827–829.
Rogers JE, Riley RG, Li SW, O'Malley ML & Thomas BL (1985) Microbial transformation of alkylpyridines in groundwater. Wat., Air, Soil Pollut. 24: 443–454.
Schwarz G, Senghas E, Erben A, Schäfer E, Lingens F & Höke H (1988) Microbial metabolism of quinoline and related compounds. I. Isolation and characterization of quinoline-degrading bacteria. System. Appl. Microbiol. 10: 185–190.
Shukla OP (1974) Microbial decomposition of α-picoline. Indian J. Biochem. Biophys. 11: 192–200.
Shukla OP (1975) Microbial decomposition of 2-ethylpyridine, 2,4-lutidine and 2,4,6-collidine. Indian. J. Exp. Biol. 13: 574–575.
Shukla OP (1986) Microbial transformation of quinoline by a Pseudomonas sp. Appl. Environ. Microbiol. 51: 1332–1342.
Shukla OP & Kaul SM (1974) A constitutive pyridine degrading system in Corynebacterium sp. Indian J. Biochem. Biophys. 11: 201–207.
Shukla OP & Kaul SM (1975) Succinate semialdehyde, an intermediate in the degradation of pyridine by Brevibacterium sp. Indian J. Biochem. Biophys. 12: 321–330.
Shulka OP (1984) Microbial transformation of pyridine derivatives. J. Sci. Ind. Res. 43: 98–116.
Sims GK & O'Loughlin EJ (1989) Degradation of pyridines in the environment. Crit. Rev. Environ. Control. 19: 309–340.
Sims GK & O'Loughlin EJ (1992) Riboflavin production during growth of Micrococcus luteus on pyridine. Appl. Environ. Microbiol. 58: 3423–3425.
Sims GK & Sommers LE (1985) Degradation of pyridine derivatives in soil. J. Environ. Qual. 14: 580–584.
Sims GK, Sommers LE & Konopka A. (1986). Degradation of pyridine by Micrococcus luteus isolated from soil. Appl. Environ. Microbiol. 51: 963–968.
Smibert RM & Krieg NR (1982) General Characterization. In: Gerhardt P (Ed), Manual of Methods for General Microbiology (pp. 433–434). American Society for Microbiology, Washington, D.C.
Stackebrandt E & Woese CR. (1979) A phylogenic dissection of the family Micrococcaceae. Curr. Microbiol. 2: 317–322.
Stuermer DH, Ng DJ & Morris CJ (1982) Organic contaminants in groundwater near an underground coal gasification site in northeastern Wyoming. Environ. Sci. Technol. 16: 582–587.
Watson GK & Cain RB (1975) Microbial metabolism of the pyridine ring. Metabolic pathways of pyridine degradation by soil bacteria. Biochem. J. 146: 157–172.
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O'Loughlin, E.J., Sims, G.K. & Traina, S.J. Biodegradation of 2-methyl, 2-ethyl, and 2-hydroxypyridine by an Arthrobacter sp. isolated from subsurface sediment. Biodegradation 10, 93–104 (1999). https://doi.org/10.1023/A:1008309026751
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DOI: https://doi.org/10.1023/A:1008309026751