Abstract
A psychrophilic bacteria, Deinococcus radiodurans, was used to construct a biosensor to be used in a flow injection system. The transducer used was an O2 electrode. The response of this cell-based electrode was studied towards a number of sugars. The temperature dependence of the electrode response correlated well with the behavior of the cells. Thus, the optimum temperature for measurement of glucose (0.55 mM) was about +5 °C. Since the organism used is psychrophilic, a response time at this low temperature is similar to what is achieved with mesophilic organisms at room temperature. This is the first biosensor constructed using a psychrophilic microorganism.
Similar content being viewed by others
References
Goldstein L (1976) Kinetic behaviour of immobilised enzyme systems. In: Mosbach K, ed. Methods in Enzymology, Vol. 44. New York: Academic Press, pp. 397-443.
Gounot AM (1991) Bacterial life at low temperature: physiological aspects and biotechnological implications. J. Appl. Bacteriol. 71: 386-397.
Hikuma M, Suzuki H, Yasuda T, Karube I, Suzuki S (1979a) Amperometric estimation of BOD by using living immobilised cells. Eur. J. Appl. Microbiol. Biotechnol. 8: 289-297.
Hikuma M, Kubo T, Yasuda T, Karube I, Suzuki S (1979b) Microbial electrode sensor for alcohols. Biotech. Bioengg. 21: 1845-1853.
Hikuma M, Kubo T, Yasuda T, Karube I, Suzuki S (1979c) Amperometric determination of acetic acid with immobilised Trichosporon brassicae. Anal. Chim. Acta 109: 33-38.
Hikuma M, Obana H, Yasuda T (1980) Amperometric determination of total assimilable sugars in fermentation broths with use of immobilised whole cells. Enzyme Microbiol. Tech. 2: 234-238.
Karube I, Mitsuda S, Suzuki S (1979) Glucose sensor using immobilised whole cells of Pseudomonas fluorescens. Eur. J. Appl. Microbiol. Biotechnol. 7: 343-350.
Matsunaga T, Karube I, Suzuki S (1978) Rapid determination ofnicotinic acid by immobilised Lactobacillus arabinosus. Anal. Chim. Acta 99: 233-239.
Marshall JC (1997) Cold-adapted enzymes. Trends Biotechnol. 15: 359-363.
Nagy G, Strop von LH, Guilbault GG (1973) Enzyme electrode for glucose based on iodide membrane sensor. Anal. Chim. Acta 66: 443-455.
Rella R, Ferrara D, Barison G, Doretti L, Lora S (1996) High temperature operating biosensor for the determination of phenol and related compounds. Biotechnol. Appl. Biochem. 24: 83-88.
Sode K, Nakasono S, Tanaka M, Matsunaga T (1993) Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase. Biotech. Bioeng. 42: 251-254.
Wiebel MK, Dritschilo W, Bright HJ, Humphrey AE (1973) Immobilised enzymes: a protype apparatus for oxidase enzymes in chemical analysis utilizing covalently bound glucose oxidase. Anal. Biochem. 52: 402-414.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nandakumar, R., Mattiasson, B. A low temperature microbial biosensor using immobilised psychrophilic bacteria. Biotechnology Techniques 13, 689–693 (1999). https://doi.org/10.1023/A:1008963730069
Issue Date:
DOI: https://doi.org/10.1023/A:1008963730069