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Overview of DNA chip technology

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Abstract

DNA chip technology utilizes microscopic arrays (microarrays) of molecules immobilized on solid surfaces for biochemical analysis. Microarrays can be used for expression analysis, polymorphism detection, DNA resequencing, and genotyping on a genomic scale. Advanced arraying technologies such as photolithograpy, micro-spotting and ink jetting, coupled with sophisticated fluorescence detection systems and bioinformatics, permit molecular data gathering at an unprecedented rate. Microarray-based characterization of plant genomes has the potential to revolutionize plant breeding and agricultural biotechnology. This review provides an overview of DNA chip technology, focusing on manufacturing approaches and biological applications.

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Authors and Affiliations

  1. Department of Plant and Soil Sciences, University of Delaware, Newark, Delaware, 19717, USA

    Bertrand Lemieux

  2. Department of Cell Biology, DLO – Center for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Asaph Aharoni

  3. Department of Biochemistry, Beckman Center, Stanford University School of Medicine, Stanford, CA, 94305, USA

    Mark Schena

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  1. Bertrand Lemieux
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  2. Asaph Aharoni
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  3. Mark Schena
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Lemieux, B., Aharoni, A. & Schena, M. Overview of DNA chip technology. Molecular Breeding 4, 277–289 (1998). https://doi.org/10.1023/A:1009654300686

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