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RAPD analysis of blast resistant somaclones from upland rice cultivar IAC 47 for genetic divergence

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Abstract

Seventeen somaclones of upland rice cultivar IAC 47 showing different plant types, and either resistance or susceptibility to leaf blast, were utilized for random amplified polymorphic DNA (RAPD) analysis. Somaclones exhibited differences in reaction to isolates of Pyricularia grisea. Two somaclones (SC02 and SC04) were resistant to all three field isolates of somaclones, while the cultivar IAC 47 was susceptible. The inheritance study of two distinct plant types, one with erect bright green leaves and the other with droopy yellow green leaves, showed that a single possibly different, dominant gene governs each plant type. Of 32 random decamer primers utilized, OPA02 and OPD02 detected polymorphisms between somaclones showing erect bright green leaves and droopy yellow green leaves. Reliable grouping exhibiting 80% similarity was achieved with 17 primers. Leaf blast resistance to race IC-2 of P. grisea was associated with the plant type of erect bright green leaves.

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Authors and Affiliations

  1. Escola de Agronomia da Universidade Federal de Goiás (UFG), Caixa Postal 131, 74001-970, Goiânia, GO, Brazil

    L.G. Araújo & L.J. Chaves

  2. Embrapa Arroz e Feijão, Caixa Postal 179, 75375-000, Santo Antônio de Goiás, GO, Brazil

    A.S. Prabhu & M.C. Filippi

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  1. L.G. Araújo
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  2. A.S. Prabhu
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  3. M.C. Filippi
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  4. L.J. Chaves
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Correspondence to L.G. Araújo.

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Araújo, L., Prabhu, A., Filippi, M. et al. RAPD analysis of blast resistant somaclones from upland rice cultivar IAC 47 for genetic divergence. Plant Cell, Tissue and Organ Culture 67, 165–172 (2001). https://doi.org/10.1023/A:1011960225472

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