Abstract
The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX ativities.
Similar content being viewed by others
References
Tiollais P, Pourcel C & Dejean A (1985) Nature 317: 489–495
Breasley RP (1988) Cancer 61: 1942–1956
Feitelson MA & Duan L-X (1997) Am. J. Pathol. 150: 1141–1157
Chen WN & Oon CJ (1999) FEBS Lett. 453: 237–242
Chen WN, Oon CJ, Leong AL, Koh S & Teng SW (2000) Biochem. Biophys. Res. Commun. 276: 885–892
Su F & Schneider RJ (1997) Proc. Natl. Acad. Sci. USA 94: 8744–8749
Kim CM, Koike K, Saito I, Miyamura T & Jay G (1991) Nature 315: 317–320
Gottlob K, Pagano S, Levrero M & Graessmann A (1998) Cancer Res. 58: 3566–3570
Koike K & Takada S (1995) Intervirology 38: 89–99
Fischer M, Runkel L & Schaller H (1995) Virus Genes 10: 99–102
Huang J, Kwong J, Sun EC-Y & Liang TJ (1996) J. Virol. 70: 5582–5591
Sirma HS, Weil R, Rosmorduc O, Urban S, Israel A, Kremsdorf D & Brechot C (1998) Oncogene 16: 2051–2063
Coux O, Tanaka K & Goldberg AL (1996) Ann. Rev. Biochem. 65: 801–847
Su Q, Schroder CH, Hofmann WJ, Otto G, Pichlmayr R & Bannasch P (1998) Hepatology 27: 1109–1120
Yerly-Motta V, Pavy JJ & Herve P (1998) Biotech. Histochem. 74: 119–128
Hu Z, Zhang Z, Doo E, Coux O, Goldberg AL & Liang TJ (1999) J. Virol. 73: 7231–7240
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, W.N., Oon, C.J. & Goo, K.S. Hepatitis B virus X protein in the proteasome of mammalian cells: defining the targeting domain. Mol Biol Rep 28, 31–34 (2001). https://doi.org/10.1023/A:1011997704112
Issue Date:
DOI: https://doi.org/10.1023/A:1011997704112