Abstract
Somatic cell counts, N-acetyl-β-D-glucosaminidase (NAGase) activity and the infection status of the udder were determined in quarter milk samples (n = 86) from 22 multiparous, clinically healthy camels, traditionally managed by Bedouin nomads in the Negev desert, Israel. Seventy (81.4%) of the 86 samples examined contained bacteria, of which 35 (40.7%) gave mixed isolations of two or more bacteria, suggesting the existence of subclinical mastitis in the camel herds studied. Sixteen samples (18.6%) yielded no growth of bacteria. Staphylococcus aureus, Micrococcus spp., Bacillus spp., Streptococcus dysgalactiae and Escherichia coli were the main organisms isolated. The somatic cell count (SCC) ranged from 1.01×105 to 11.78×106 cells/ml. NAGase values were between 41.4 and 372 NAGase units. Quarter milk samples that contained bacteria had significantly (p<0.01) higher mean values for SCC but the mean NAGase levels were not significantly different for the bacteriologically negative and positive samples. There was a low correlation coefficient (r 2 = 0.097) between the SCC and NAGase in the quarter milk samples from which bacteria were not isolated (n = 16) and a low negative correlation (r 2 = –0.038) with the samples that contained bacteria (n = 70). The type of bacteria had a significant effect (p<0.01) on the SCC but not on the NAGase activity. Quarter samples from which Staphylococcus aureus (coagulase positive) was isolated showed the highest mean SCC and this organism is therefore suspected to be the underlying cause of the subclinical mastitis. The SCC gave a better indication of the presence of pathogenic microorganisms in milk samples than did NAGase.
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REFERENCES
Abdurahman, O.A.S., 1995. N-acetyl-β-D-glucosaminidase and serum albumin as indicators of subclinical mastitis in the camel. Journal of Veterinary Medicine A, 42, 643-647
Abdurahman, O.A.S., 1996. The detection of sub-clinical mastitis in the bactrian camel (Camelus bactrianus) by somatic cell count and California mastitis test. Veterinary Research Communications, 20, 9-14
Abdurahman, O.A.S., Cooray, R. and Bornstein, S., 1992. The ultrastructure of cell fragments in mammary secretions of Camelus bactrianus. Journal of Veterinary Medicine A, 39, 648-655
Abdurahman, O.A.S., Ageb, H., Abbas, B. and Astron, G., 1995. Relations between udder infection and somatic cells in camel (Camelus dromedarius) milk. Acta Veterinaria Scandinavica, 36, 423-431
American Public Health Association, 1985. Standard Methods for the Examination of Dairy Products, 15th edn, (American Public Health Association, Washington, DC)
Barbour, E.K., Nabbut, N.H., Frerichs, W.M., Al-Nakhli, H.M. and Al-Mukayel, A.A., 1985. Mastitis in Camelus dromedarius in Saudi Arabia. Tropical Animal Health and Production, 17, 173-179
Booth, J.M., 1995. Progress in control of mastitis. In: A. Saran and S. Soback (eds), Proceedings of the 3rd International Mastitis Seminar, Tel Aviv (Israel), (International Dairy Federation; Book II-Session 4), 3-9
Brown, R.W., Morse, G.E., Newbould, F.H.S. and Slanetz, L.W., 1969. Microbiological Procedures for the Diagnosis of Bovine Mastitis, (National Mastitis Council Inc., Washington DC)
Kitchen, B.J., Middleton, G. and Salmon, M., 1978. Bovine milk N-acetyl-β-D-glucosaminidase and its significance in detection of abnormal udder infection. Journal of Dairy Research, 45, 15-20
Kitchen, B.J., Seng Kwee, W., Middleton, G. and Andrews, R.J., 1984. Relationship between the level of N-acetyl-β-D-glucosaminidase (NAGase) in bovine milk and the presence of mastitis pathogens. Journal of Dairy Research, 51, 11-16
Kospakov, Z.K., 1978. Cell content in the milk of bactrian camels, depending on stage of lactation and condition of the udder. Veterinary Bulletin, 48, Abstract 6960
Maisi, P., Junttila, J. and Seppanen, J., 1987. Detection of sub-clinical mastitis in ewes. British Veterinary Journal, 143, 402-408
Minitab, 1989. Data analysis software, release 7.2-standard version, Minitab, Inc.
Obeid, A.I. and Bagadi, H.O., 1996. Mastitis in Camelus dromedarius and the somatic cell count of camels' milk. Research in Veterinary Science, 61, 55-58
Schalm, O.W., Carroll, E.J. and Jain, N.C., 1971. Bovine Mastitis, (Lea and Febiger, Philadelphia), 315-329
Schwartz, H.J. and Dioli, M., 1992. The One-humped Camel in Eastern Africa: A Pictorial Guide to Diseases, Health Care and Management, (Verlag Joseph Margraf, Germany)
Timms, L.L. and Schultz, L.H., 1985. N-acetyl-β-D-glucosaminidase activity and somatic cells in goat milk. Journal of Dairy Science, 68, 3363-3366
Yagil, R., 1982. Camels and Camel Milk, (FAO Animal Production and Health Paper, Rome)
Yagil, R., 1985. The Desert Camel: Comparative Physiological Adaptation, Comparative Animal Nutrition 5, (Karger, Basel)
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Guliye, A., Van Creveld, C. & Yagil, R. Detection of Subclinical Mastitis in Dromedary Camels (Camelus dromedarius) using Somatic Cell Counts and the N-Acetyl-β-D-glucosaminidase Test. Tropical Animal Health and Production 34, 95–104 (2002). https://doi.org/10.1023/A:1014324421258
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DOI: https://doi.org/10.1023/A:1014324421258