Abstract
Electron transfer in the reaction center of the purple photosynthetic bacterium Rb. sphaeroides R-26 has been studied at room temperature by transient absorption spectroscopy with a time resolution of 120 fs. Measurements of absorption changes were performed in the range from 400 nm up to 680 nm, after excitation with a laser pulse of 80 fs duration within the absorption band of the bacteriochlorophyll at 800 nm. The excited state of the primary donor, characterized by the absorbance changes extending over the whole spectral range investigated, appeared within 120 fs and gave rise to the bleaching of the Qx absorption band of bacteriochlorophyll at 600 nm, increased further by electron transfer to bacteriopheophytin in ∼3 ps. Photoreduction of the bacteriopheophytin acceptor detected at 546 nm and 670 nm proceeded with the same time constant. Multiphase absorbance changes were relatively the largest in the blue spectral range between 415 nm and 450 nm. Apart from the immediate absorbance increase due to excitation of the primary donor, another fast increasing phase was detected characterized by a wavelength dependent time constant—from ∼5.5 ps at 415 nm to ∼1.9 ps at 450 nm. Both the photooxidised primary donor and photoreduced bacteriopheophytin contributed to the amplitude of this phase. The electron transfer from the reduced bacteriopheophytin to a quinone acceptor was observed as a decrease in the intensity of the transient absorption bands at about 422 nm and at 670 nm, with disappearance of the bleaching at 546 nm and increase of the bleaching at 600 nm, all in ∼200 ps.
Similar content being viewed by others
REFERENCES
D. W. Reed and R. K. Clayton (1968) Biochem. Biophys. Res. Commun. 30, 471-475.
J. Breton and A. Vermeglio (1982) in Govindjee (Ed.), Photosynthesis: Energy Conversion by Plants and Bacteria, New York, pp. 153-194.
A. J. Hoff (1982) Biophys. Struct. Mech. 8, 107-150.
C. Kirmaier and D. Holten (1987) Photosynth. Res. 13, 225-260.
A. J. Hoff and J. Deisenhofer (1997) Phys. Rep. 287, 1-247.
C.-H. Chang, D. Tiede, J. Tang, U. Smith, J. Norris, and M. Schiffer (1986) FEBS Lett. 205, 82-86.
J. P. Allen, G. Feher, T. O. Yeates, H. Komiya, and D. C. Rees (1987) Proc. Natl. Acad. Sci. USA 84, 5730-5734.
D. Holten, M. W. Windsor, W. W. Parson, and J. P. Thomber (1978) Biochim. Biophys. Acta 501, 112-126.
C. Kirmaier, D. Holten, and W. W. Parson (1985) Biochim. Biophys. Acta 810, 33-48.
K. Dressler, E. Umlauf, S. Schmidt, P. Hamm, W. Zinth, S. Buchman, and H. Michel (1991) Chem. Phys. Lett. 183, 270-276.
M. Volk, A. Ogrodnik, and M. E. Michel-Beyerle (1995) in R. E. Blankenship, M. T. Madigan, and C. E. Bauer (Eds.), Anoxigenic Photosynthetic Bacteria, Kluwer Academic, Dordrecht, The Netherlands, p. 595.
C. A. Wright and R. K. Clayton (1974) Biochim. Biophys. Acta 33, 246-260.
A. J. Campillo, R. C. Hyer, T. G. Monger, W. W. Parson, and S. L. Shapiro (1977) Proc. Natl. Acad. Sci. USA 74, 1997-2001.
J.-L. Martin, J. Breton, A. J. Hoff, A. Migus, and A. Antonetti (1986) Proc. Natl. Acad. Sci. USA 83, 957-961.
W. Holtzapfel, U. Finkele, W. Kaiser, D. Oersterhelt, H. Scheer, H. U. Stilz, and W. Zinth (1989) Chem. Phys. Lett. 160, 1-7.
W. Holtzapfel, U. Finkele, W. Kaiser, D. Oersterhelt, H. Scheer, H. U. Stilz, and W. Zinth (1990) Proc. Natl. Acad. Sci. USA 87, 5168-51727.
A. Dobek, J. Deprez, G. Paillotin, W. Leibl, H.-W. Trissl, and J. Breton (1990) Biochim. Biophys. Acta 1015, 313-321.
J. Breton, J.-L. Martin, A. Migus, A. Antonetti, and A. Orszag (1986) Proc. Natl. Acad. Sci. USA 83, 5121-5125.
G. Gingras (1978) in R. K. Clayton and W. R. Sistrom (Eds.), The Photosynthetic Bacteria, Plenum Press, New York, pp. 119-131.
G. Berger, D. Tiede, and J. Breton (1984) Biochem. Biophys. Res. Commun. 121, 47-54.
R. Naskręcki, M. Lorenc, M. Ziółek, J. Karolczak, J. Kubicki, A. Maciejewski, and M. Szymański (1999) Bull. Polish Acad. Sci. Chem. 47, 333-346.
C. Kirmaier and D. Holten (1990) Proc. Natl. Acad. Sci. USA 87, 3552-3556.
L. Slooten (1972) Biochim. Biophys. Acta 275, 208-218.
A. Vermeglio and R. K. Clayton (1977) Biochim. Biophys. Acta 461, 159-165.
W. Parson (1978) in R. K. Clayton and W. R. Sistron (Eds.), The Photosynthetic Bacteria, Plenum Press, New York and London, pp. 455-469.
J. Jortner and M. E. Michel-Beyerele (1985) in M. E. Michel-Beyerele (Ed.), Antennas and Reaction Centers of Photosynthetic Bacteria, Springer Series in Chemical Physics, Vol. 42, Springer, Berlin, p. 344.
M. Bixon, J. Jortner, M. Plato, and M. E. Michel-Beyerele (1988) in J. Breton and A. Vermeglio (Eds.), Photosynthetic Bacterial Reaction Center—Structure and Dynamics, Plenum Press, New York, p. 399.
R. A. Marcus and N. Sutin (1985) Biochim. Biophys. Acta 811, 265-322.
S. F. Fischer and P. O. J. Scherer (1987) Chem. Phys. 115, 151-158.
W. W. Parson, A. Warshel, C. Creighton, and J. R. Norris (1988) in J. Breton and A. Vermeglio (Eds.), The Photosynthetic Bacterial Reaction Center—Structure and Dynamics, Plenum Press, New York, p. 309.
M. E. van Brederode and R. van Grondelle (1999) FEBS Lett. 455, 1-7.
T. S. Takeuchi and J. P. Thomber (1994) Aust. J. Plant Physiol. 21, 759-770.
V. Barzda, A. Istokovics, L. Simidijev, and G. Garab (1996) Biochemistry 35, 8981-8985.
Z. Cseh, S. Rajagopal, T. Tsonev, M. Busheva, E. Papp, and G. Garab, submitted for publication.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gibasiewicz, K., Naskrecki, R., Ziółek, M. et al. Electron Transfer in the Reaction Center of the Photosynthetic Bacterium Rb. sphaeroides R-26 Measured by Transient Absorption in the Blue Spectral Range. Journal of Fluorescence 11, 33–40 (2001). https://doi.org/10.1023/A:1016695515341
Issue Date:
DOI: https://doi.org/10.1023/A:1016695515341