Abstract
The human central CB1 and peripheral CB2 cannabinoid receptors were expressed in Escherichia coli as fusion proteins with the maltose-binding protein at their amino-termini and a hexa-histidine/Flag tag at their carboxyl-termini. Western blot analysis of the expressed proteins revealed considerable degradation of the CB1 fusion, which failed to bind either the cannabinoid agonist CP 55,940 or the CB1-specific antagonist SR 141716A. In contrast, the CB2 fusion was well-expressed and bound several cannabinoids with affinities comparable to those observed in mammalian expression systems.
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Calandra, B., Tucker, J., Shire, D. et al. Expression in Escherichia coli and characterisation of the human central CB1 and peripheral CB2 cannabinoid receptors. Biotechnology Letters 19, 425–428 (1997). https://doi.org/10.1023/A:1018383808401
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DOI: https://doi.org/10.1023/A:1018383808401