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CMA3-banding pattern and fluorescence in situ hybridiz ation with 18S rRNA genes in zebrafish chromosomes

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Abstract

This study provides new data on zebrafish chromosomes, obtained from the chromomycin A3-banding pattern and mapping of18S rRNA genes by fluorescence in situ hybridization (FISH). C-banding and Ag-staining were also performed to analyse whether variation in heterochromatin and Ag-nucleolus organizer regions (NORs) exists among various commercially purchased strains. The results provide information on heterochromatin composition and on the existence of inter individual NOR polymorphism and contribute to the construction of an idiogram suitable for gene mapping.

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Authors and Affiliations

  1. Department of Animal and Human Biology, University of Rome 1, via A. Borelli 50, 00161, Rome, Italy

    Ekaterina Gornung, Ivan Gabrielli & Luciana Sola

  2. Department of Biology, University of Rome 2, via della Ricerca Scientifica, 00133, Rome, Italy

    Stefano Cataudella

Authors
  1. Ekaterina Gornung
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  2. Ivan Gabrielli
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  3. Stefano Cataudella
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  4. Luciana Sola
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Gornung, E., Gabrielli, I., Cataudella, S. et al. CMA3-banding pattern and fluorescence in situ hybridiz ation with 18S rRNA genes in zebrafish chromosomes. Chromosome Res 5, 40–46 (1997). https://doi.org/10.1023/A:1018441402370

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  • DOI: https://doi.org/10.1023/A:1018441402370

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