Abstract
Age-related changes of IgG N-linked oligosaccharides isolated from normal human serum are reported for 403 individuals (male 227 and female 176), varying in age from 0 to 85 years. The IgG N-linked oligosaccharides were released from the protein by digestion with a glycoamidase and reductively aminated with the fluorescent reagent, 2-aminopyridine. The mixture of pyridylaminated oligosaccharides was separated at high resolution by HPLC using a reverse-phase column. From the results of neutral oligosaccharide analysis, agalactosyl glycoform and bisecting GlcNAc-containing glycoform were shown to increase with increasing age. Spearman's correlation coefficients were 0.503 and 0.473, respectively. Thus, in healthy people, an increase of both types of glycoforms correlates weakly with age. In addition, differences were demonstrated between male and female groups in their twenties. The quantity of agalactosyl glycoform was found to be lower in females than in males. No significant differences, however, were observed in the quantity of bisecting GlcNAc-containing glycoforms between males and females. Abbreviations: Gal, D-galactose: GlcNAc, N-acetyl-D-glucosamine; Man, D-mannose; Fc, C-terminal half of the heavy chain dimers of IgG; HPLC, high-performance liquid chromatography; IgG, immunoglobulin G; ODS, octadecylsilyl; PA, pyridylamino
Similar content being viewed by others
References
Takahashi N, Ishii I, Ishihara H, Mori M, Tejima S, Jefferis R, Endo S, Arata Y (1987) Biochemistry 26: 1137–44.
Takahashi N, Nakagawa H, Fujikawa K, Kawamura Y, Tomiya N (1995) Anal Biochem 226: 139–46.
Parekh RB, Dwek RA, Sutton BJ, Fernandes DL, Leung JA, Stanworth D, Rademacher TW, Mizuochi T, Taniguchi T, Matsuta K, Takeuchi F, Nagano Y, Miyamoto T, Kobata A (1985) Nature 316: 452–57.
Ito K, Takahashi N, Hirayama M, Honda H, Takahashi A (1993) J Clin Biochem Nutr 14: 61–69.
Parekh RB, Roitt I, Isenberg D, Dwek RA, Rademacher TW (1988) J Exp Med 167: 1731–36.
Tsuchiya N, Endo T, Matsuta K, Yoshinoya S, Takeuchi F, Nagano Y, Shiota M, Furukawa K, Kochibe N, Ito K, Kobata A (1993) J Immunol 151: 1137–46
Yamamoto S, Hase S, Fukuda S, Sano O, Ikenaka T (1989) J Biochem 105: 547–55.
Kibe T, Fujimoto S, Ishida C, Togari H, Wada Y, Okada S, Nakagawa H, Tsukamoto Y, Kawamura Y. Takahashi N (1996) J Clin Biochem Nutr 21: 57–63.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamada, E., Tsukamoto, Y., Sasaki, R. et al. Structural changes of immunoglobulin G oligosaccharides with age in healthy human serum. Glycoconj J 14, 401–405 (1997). https://doi.org/10.1023/A:1018582930906
Issue Date:
DOI: https://doi.org/10.1023/A:1018582930906