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Operational and storage stability of neutral β-mannanase from Bacillus licheniformis

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Abstract

The stability of neutral β-mannanase from Bacillus licheniformis during operation and storage was investigated. The enzyme activity decreased by 70% with a hydrolysate of glucomannan at 20 g l−1 over 30 min at 25 °C. In an enzymatic membrane reactor operated at 50 °C after 24 h, the loss of enzyme activities were 23% and 9% in the absence/presence of the substrate. The residual activities of the enzyme were 21% and 90%, respectively, when stored in 30% (v/v) glycerol solution and in solid state at 4 °C after one year.

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Zhang, J., He, M. & He, Z. Operational and storage stability of neutral β-mannanase from Bacillus licheniformis . Biotechnology Letters 24, 1611–1613 (2002). https://doi.org/10.1023/A:1020332011421

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  • DOI: https://doi.org/10.1023/A:1020332011421

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