Abstract
Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When glucose was kept at 2.5 g l−1, cell growth and PHB synthesis were limited due to the shortage of carbon source but a higher PHB content occurred in the cell-growth stage. This shows that a low glucose concentration is favorable for PHB accumulation in R. eutropha. PHB obtained with glucose at 9 g l−1 is 1.6 times that obtained with 40 g l−1. When glucose was in the range of 9 to 40 g l−1, PHB concentration and productivity decreased significantly with the increase of glucose concentration. The highest PHB productivity was obtained with glucose at 9 g l−1.
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Shang, L., Jiang, M. & Chang, H.N. Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations. Biotechnology Letters 25, 1415–1419 (2003). https://doi.org/10.1023/A:1025047410699
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DOI: https://doi.org/10.1023/A:1025047410699