Abstract
In order to test whether 18S rDNA can influence positively GUS gene transient expression in the red alga Porphyra yezoensis, a targeting vector pQD-GUS was constructed containing a portion of the 18S rDNA of P. yezoensis and transformed it into the same strain protoplasts. The results showed that GUS protein activity was increased markedly with pQD-GUS compared to the parent pBS-GUS. It is suggested that this system can be used to enhance the expression of exogenous genes in transgenic P. yezoensis.
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Liu, H., Yu, W., Dai, J. et al. Increasing the transient expression of GUS gene in Porphyra yezoensis by 18S rDNA targeted homologous recombination. Journal of Applied Phycology 15, 371–377 (2003). https://doi.org/10.1023/A:1026083920865
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DOI: https://doi.org/10.1023/A:1026083920865