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Helicobacterpylori in Dental Plaque (A Comparison of Different PCR Primer Sets)

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Abstract

This study was designed to compare differentprimer sets for PCR analysis of H. pylori in the sameseries of 40 dental plaque samples. Three pairs ofprimers, HPU1/HPU2, HP1/HP2, and EHC-U/EHC-L, directed to the urease A gene, 16S rRNA gene, or 860-bpDNA of H. pylori, respectively, were used. Our resultsdemonstrate that EHC-L/EHC-U were moRespecific andsensitive for H. pylori added to saliva or dental plaque than HPU1/HPU2 and HP1/HP2. Thedetection rates for H. pylori DNA in dental plaquesamples from randomly selected adult patients from theDental Clinic of the University of Ulm were 26.5% (9/34) for HPU1/HPU2, 78.9% (30/38) for HP1/HP2, and100% (40/40) for EHC-U/EHC-L (P < 0.001). Nested PCRusing primers directed to the 860-bp DNA of H. pylorifurThe r confirmed the presence of H. pylori DNA (40/40) in all these samples. Our resultsindicate that primers EHC-U/EHC-L are to be recommendedfor PCR detection of H. pylori in the oralcavity.

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REFERENCES

  1. Goodwin CS, Mendall MM, North field TC: Helicobacterpylori infection. Lancet 349:265-269, 1997

    Google Scholar 

  2. Li C, Musich PR, Ha T, Ferguson DA Jr, Patel NR, Chi DS, Thomas E: High prevalence of Helicobacterpylori in saliva demonstrated by a nove l PCR assay. J Clin Pathol 48:662-666, 1995

    Google Scholar 

  3. Majmudar P, Shah SM, Dhunjibhoy KR, Desai HG: Isolation of Helicobacterpylori from dental plaques in healthy volunteers. Indian J Gastroenterol 9:271-272, 1990

    Google Scholar 

  4. Banatvala N, Lopez CR, Owen R, Abdi Y, Davis G, Hardie J, Feldman R: Helicobacterpylori in dental plaque. Lancet 341:380, 1993

    Google Scholar 

  5. Ferguson DA Jr, Li C, Patel NR, Mayberry WR, Chi DS, Thomas E: Isolation of Helicobacterpylori from saliva. J Clin Microbiol 31:2802-2804, 1993

    Google Scholar 

  6. Pytko-Polonczyk J, Konturek SJ, Karcze wska E, Bielanski W, Kaczmarczyk-Stachows ka A: Oral cavity as permanent reservoir of Helicobacterpylori and potential source of reinfection. J Physiol Pharmacol 47:121-129, 1996

    Google Scholar 

  7. Shames B, Krajden S, Fuksa M, Babida C, Penner JL: Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque. J Clin Microbiol 27:2849-2850, 1989

    Google Scholar 

  8. Song QS, Zheng ZT, Yu H: Helicobacterpylori in dental plaque. Chin J Internal Med 33:459-461, 1994

    Google Scholar 

  9. Bernander S, Dalen J, Gastrin B, Hedenborg L, Lamke LO, Obrn R: Absence of Helicobacterpylori in dental plaque in Helicobacterpylori positive dyspeptic patients. Eur J Clin Microbiol Infect Dis 12:282-285, 1993

    Google Scholar 

  10. Bickley J, Owen RJ, Fraser AG, Pounder RE: Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacterpylori in gastric biopsy sample s and dental plaque. J Med Microbiol 39:338-344, 1993

    Google Scholar 

  11. Hardo PG, Tugnait A, Hassan F, Lynch DAF, West AP, Mapstone NP, Quirke P, Chalmers DM, Kowolik MJ, Axon ATR: Helicobacterpylori infection and dental care. Gut 37:44-46, 1995

    Google Scholar 

  12. Luman W, Alkout AM, Blackwell CC, Weir DM, Palmer KR: Helicobacterpylori in the mouthÐ negative isolation from dental plaque and saliva. Eur J Gastroenterol Hepatol 8:11-14, 1996

    Google Scholar 

  13. Von Re cklinghausen G, Weischer T, Ansorg R, Mohr C: No cultural detection of Helicobacterpylori in dental plaque. Zentralbl Bakte riol 281:102-106, 1994

    Google Scholar 

  14. Clayton CL, Kleanthous H, Coate s PJ, Morgan DD, Tabaqchali S. Sensitive detection of Helicobacterpylori by using polymerase chain reaction. J Clin Microbiol 30:192-200, 1992

    Google Scholar 

  15. Ho SA, Hoyle JA, Lewis FA, Secker AD, Cross D, Mapstone NP, Dixon MF, Wyatt JI, Tompkins DS, Taylor GR, Quirke P: Direct polymerase chain reaction test for detection of Helicobacterpylori in humans and animals. J Clin Microbiol 29:2543-2549, 1991

    Google Scholar 

  16. Asikainen S, Chen C, Slots J: Absence of Helicobacterpylori in subgingival samples determined by polymerase chain reaction. Oral Microbiol Immunol 9:318, 1994

    Google Scholar 

  17. Cammarota G, Tursi A, Montalto M, Papa A, Veneto G, Bernardi S, Boari A, Colizzi V, Fedeli G, Gasbarrini G: Role of dental plaque in the transmission of Helicobacterpylori infection. J Clin Gastroenterol 22:174-177, 1996

    Google Scholar 

  18. Li C, Ha T, Ferguson DA Jr, Chi DS, Zhao R, Patel NR, Krishnaswamy G, Thomas E: A newly developed PCR assy of H. pylori in gastric biopsy, saliva, and feces. Evidence of high prevalence of H. pylori in saliva supports oral transmission. Dig Dis Sci 41:2142-2149, 1996

    Google Scholar 

  19. Mapstone NP, Lynch DAF, Lewis FA, Axon ATR, Tompkins DS, Dixon MF, Quirke P: Identification of Helicobacterpylori DNA in the mouths and stomachs of patients with gastritis using PCR. J Clin Pathol 46:540-543, 1993

    Google Scholar 

  20. Namavar F, Roosendaal R, Kuipers EJ, Groot P de, Bijl MW, Pera AS, Graaff J de: Presence of Helicobacterpylori in the oral cavity, esophagus, stomach and faeces of patients with gastritis. Eur J Clin Microbiol Infect Dis 14:3, 1995

    Google Scholar 

  21. Nguyen AMH, Engstrand L, Genta RM, Graham DY, El-Zaatari FAK: detection of Helicobacterpylori in dental plaque by reve rse transcription-polymerase chain reaction. J Clin Microbiol 31:783-787, 1993

    Google Scholar 

  22. Olsson K, Wadstrom T, Tyszkiewicz T: H. pylori in dental plaque. Lance t 341:956-957, 1993

    Google Scholar 

  23. Shimada T, Ogura K, Ota S, Terano A, Takahashi M, Hamada E, Omata M, Sumino S, Sassa R: Identification of Helicobacterpylori in gastric specimens gastric juice, saliva, and faeces of Japanese patients. Lancet 343:1636-1637, 1994

    Google Scholar 

  24. Li C, Ferguson DA Jr, Ha T, Chi DS, Thomas E: A highly specific and sensitive DNA probe derived from chromosomal DNA of Helicobacterpylori is useful for typing H. pylori isolates. J Clin Microbiol 31:2157-2162, 1993

    Google Scholar 

  25. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning, A Laboratory Manual. New York, Cold Spring Harbor Laboratory, 1989

    Google Scholar 

  26. Kwok S, Higuchi R: Avoiding false positives with PCR. Nature 339:237-238, 1989

    Google Scholar 

  27. Yang HT: Nested-polymerase chain reaction in detection of Helicobacterpylori in human dental plaque. Chun Hua I Hsueh Tsa Chih 73:750-752, 1993

    Google Scholar 

  28. Innis MA, Gelfand DH, Sninsky JJ, White TJ: PCR Protocols, A Guide to Methods and Applications. San Diego, Academic Press, 1990

    Google Scholar 

  29. Chong SKF, Lou Q, Fitzgerald JF, Lee CH: Evaluation of 16S rRNA gene PCR with prime rs Hp1 and Hp2 for detection of Helicobacterpylori. J Clin Microbiol 34:2728-2730, 1996

    Google Scholar 

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Authors
  1. Qunsheng Song
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  2. Bernd Haller
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  3. Roland M. Schmid
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  4. Guido Adler
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  5. Gunter Bode
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Song, Q., Haller, B., Schmid, R.M. et al. Helicobacterpylori in Dental Plaque (A Comparison of Different PCR Primer Sets). Dig Dis Sci 44, 479–484 (1999). https://doi.org/10.1023/A:1026680618122

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  • DOI: https://doi.org/10.1023/A:1026680618122

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