Abstract
β-Galactosidase and streptokinase expression was tested under the control of the T7 promoter in batch and fed-batch cultures. An Escherichia coli host GJ1158, which contained the T7 RNA polymerase gene under the osmo-responsive proUp promoter, was used for expression studies. β-Galactosidase expression was enhanced from 26 mg l−1 to 127 mg l−1 in batch culture when a combination of sucrose and sorbitol was used instead of salt as an inducer. Similarly in fed-batch cultures 140 mg streptokinase l−1 was formed with sucrose and sorbitol induction which was higher than that achieved with IPTG induced cultures.
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Pal, Y., Gupta, J.C. & Mukherjee, K. Optimizing recombinant protein expression in the T7 system under the control of the proUp promoter. Biotechnology Letters 23, 41–46 (2001). https://doi.org/10.1023/A:1026712310154
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DOI: https://doi.org/10.1023/A:1026712310154