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A two-site immunometric assay for the detection of the expression level of α2(C2)-adrenergic receptor produced in Escherichia coli

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Abstract

Human α2(C2)-adrenergic receptor was expressed in Escherichia coli as a fusion protein with Bacillus circulans var. alcalophilus cyclomaltodextrin glucanotransferase. For the determination of the expression level (0.6 mg of solubilized fusion protein l−1 of E. coli culture), a two-site immunometric assay based on two monoclonal antibodies with different epitopes was developed.

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Authors and Affiliations

  1. Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, P.O. Box 123, FIN-20521 , Turku, Finland

    Heini Frang & Jukka Hellman

  2. Department of Pharmacology and Clinical Pharmacology, and Medicity Research Laboratory, University of Turku, Tykistökatu 6, FIN-20520 , Turku, Finland

    Heini Frang & Mika Scheinin

  3. Institute of Biotechnology, University of Helsinki, P.O. Box 56, FIN-00014

    Adrian Goldman

  4. University of Helsinki, Finland

    Adrian Goldman & Tuomo Glumoff

Authors
  1. Heini Frang
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  2. Jukka Hellman
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  3. Adrian Goldman
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  4. Mika Scheinin
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  5. Tuomo Glumoff
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Frang, H., Hellman, J., Goldman, A. et al. A two-site immunometric assay for the detection of the expression level of α2(C2)-adrenergic receptor produced in Escherichia coli. Biotechnology Letters 22, 1963–1966 (2000). https://doi.org/10.1023/A:1026766330471

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  • DOI: https://doi.org/10.1023/A:1026766330471

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