Abstract
Studies have been conducted to ascertain the preventive effect of pyruvate against diabetes induced damage to DNA and associated morphogenetic changes in the mouse lens. Such changes were characterized by DNA nicks as well as by gross morphological changes in the nuclei, evident respectively by TUNEL and Hoechst staining procedures. Morphogenetic changes were also apparent by abnormal diferentiation of the germinal epithelial cells and errors in their migratory pathway. These changes were prevented by simultaneous administration of pyruvate to the diabetic animals. The preventive effect of this agent is attributable to its property of scavenging oxy-radicals generated by high levels of the sugars. (Mol Cell Biochem 262: 233–237, 2004)
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References
Caird IF, Hutchinson M, Pirie A: Cataract extraction and diabetes. Br J Ophthalmol 49: 466–471, 1964
Hiller R, Kahn H: Senile cataract extraction and diabetes: Br J Ophthalmol 60: 282–286, 1976
Kirby DB: Cataract and diabetes. Arch Ophthalmol 9: 966–973, 1933
Varma SD, Kumar S, Richards RD: Light-induced damage to ocular lens cation pump: Prevention by vitamin C Proc Natl Acad Sci USA 76: 3504–3506, 1979
Devamanoharan PS, Henein M, Morris S, Ramachandran S, Richards RD, Varma SD: Prevention of selenite cataract by vitamin C. Exp Eye Res 52: 563–568, 1991
Trevithick JR, Mitton KP: Vitamins C and E in cataract risk reduction. Int Ophthalmol Clin 40(4): 59–69, 2000
AnsariNH, Srivastava SK: Allopurinol promotes and butylated hydroxyl toluene prevents sugar-induced cataractogenesis. Biochem Biophys Res Commun 168(3): 939–943, 1990
ZhaoW, Devamanoharan PS, Henein M, Ali AH, Varma SD: Diabetes-induced biochemical changes in rat lens: Attenuation of cataractogenesis by pyruvate. Diabetes Obes Metab 2: 165–174, 2000
Hegde KR, Henein MG, Varma SD: Mouse model for diabetic cataract; Biochemical studies: Preventive effect of pyruvate. Annual Meeting of Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida, 2002
Hegde KR, Henein MG, Varma SD: Establishment of the mouse as a model animal for the study of diabetic cataracts. Ophthalmic Res 35: 12–18, 2003
Takamura Y, Sugimoto Y, Kubo E, Takahashi Y, Akagi Y: Immunohistochemical study of apoptosis of lens epithelial cells in human and diabetic rat cataracts. Jpn J Ophthalmol 45: 559–563, 2001
Van Heyningen R: Formation of polyols by the lens of the rat with sugar cataracts. Nature 164: 194–196, 1959
Varma SD, Chand D, Sharma YR, Kuck JF, Richards RD: Oxidative stress on lens and cataract formation. Role of light and oxygen. Curr Eye Res 3: 35–57, 1984
Hegde KR, Henein MG, Varma SD: Establishment of mouse as an animal model for study of diabetic cataracts: Biochemical studies. Diabetes Obes Metab 5: 113–119, 2003
Gavrieli Y, Sherman Y, Ben-Sasson SA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119(3): 493–501, 1992
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Hegde, K., Varma, S. Morphogenetic and apoptotic changes in diabetic cataract: Prevention by pyruvate. Mol Cell Biochem 262, 233–237 (2004). https://doi.org/10.1023/B:MCBI.0000038220.47170.d0
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DOI: https://doi.org/10.1023/B:MCBI.0000038220.47170.d0