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Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells

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Abstract

The vanilloid receptor 1(VR1) is a nonselective cation channel that is activated by pungent vanilloid compound, extracellular protons, or noxious heat. mRNA of VR1 and vanilloid receptor 1–like receptor (VRL1) were expressed in PC12 cells, and only VR1 mRNA was detected in glioma and A10 cell lines. VR1 protein was demonstrated in PC12 cells by immunocytochemistry and Western blotting. Capsaicin (CPS), the VR1 receptor agonist, led to an increase in intracellular calcium ion, and this effect was blocked by pretreatment with VR1 receptor antagonist capsazepin (CPZ). Treatment of PC12 cells with low concentration of CPS (5–50 μM) increased reactive oxygen species (ROS) production, and inducible nitric oxide synthase (iNOS) was expressed after CPS treatment for 24 h. These CPS-induced changes are inhibited by pretreatment of CPZ. These findings suggest that CPS-induced iNOS expression through the VR1 and/or VRL1-mediated pathway, and this may explain the CPS-mediated physiological and pathological effects in neuron system.

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Authors and Affiliations

  1. Department of Biochemistry, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan

    Shanlou Qiao, Weihua Li, Ryoko Tsubouchi, Keiko Murakami & Masataka Yoshino

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  1. Shanlou Qiao
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Qiao, S., Li, W., Tsubouchi, R. et al. Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells. Neurochem Res 29, 687–693 (2004). https://doi.org/10.1023/B:NERE.0000018839.59457.5c

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