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Larvicidal activity of crystal-forming strains of Brevibacillus laterosporus

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Abstract

The optimum conditions for growth, sporulation, and crystal-formation in four isolated crystal-forming strains of Bacillus laterosporus were determined. It was shown that culture broth and pellets of bacterial culture liquid possess larvicidal activity against larvae of mosquitoes A. stephensi and A. aegypti. The protein nature of crystal was shown. Crystals are monocomponent containing a protein with MM of 68 or 130 kDa. Purified protein crystals demonstrated larvicidal activity. Specific larvicidal activity of crystals of various strains essentially differed. High larvicidal activity of B. laterosporus strains allows for them to be recommended as producers of antimosquito biological preparations.

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Abbreviations

DTT:

dithiotreitol

DFP:

diisopropylfluorophosphate

CL:

cultural liquid

CFU:

colony-forming unit

OD:

optical density

PAAG:

polyacrylamidegel

PMSF:

phenylmethylsolfonylfluoride

EDTA:

ethylendiaminetetraacetic acid

SDS:

sodium dodecylsulphat

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    M. V. Zubasheva, T. A. Smirnova & R. R. Azizbekyan

  2. Martsinovskii Institute for Parasitology and Tropical Medicine, Sechenov Moscow Medical Academy, Moscow, 119435, Russia

    L. A. Ganushkina

Authors
  1. M. V. Zubasheva
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  2. L. A. Ganushkina
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  3. T. A. Smirnova
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  4. R. R. Azizbekyan
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Correspondence to M. V. Zubasheva.

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Original Russian Text © M.V. Zubasheva, L.A. Ganushkina, T.A. Smirnova, R.R. Azizbekyan, 2009, published in Biotekhnologiya, 2009, No. 6, pp. 46–56.

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Zubasheva, M.V., Ganushkina, L.A., Smirnova, T.A. et al. Larvicidal activity of crystal-forming strains of Brevibacillus laterosporus . Appl Biochem Microbiol 46, 755–762 (2010). https://doi.org/10.1134/S0003683810080041

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