Abstract
A novel (R)-1-phenylethanol dehydrogenase was successfully purified from Lysinibacillus sp. NUST506 by preparative polyacrylamide gel electrophoresis. The enzyme is a NAD+-dependent oxidoreductase. The molecular weight of the (R)-1-phenylethanol dehydrogenase measured by SDS-PAGE was about 28 kDa. Furthermore, the optimal reaction conditions for the oxidative reaction were 70°C and pH 9.5 and for the reductive reaction were 65°C and pH 6.5. Under the optimal conditions, the KM and kcat values with (R)-1-phenylethanol as a substrate were found to be 0.78 mM and 123 s–1 and with acetophenone they were 0.56 mM and 125 s–1, respectively. The (R)-1-phenylethanol dehydrogenase became more stable at pH 9.5 in comparison with pH 5.0 and high stability was noticed at 4 and 37°C. Properties of the enzyme place it as a promising candidate for industrial applications.
Similar content being viewed by others
References
Abate, A., Brenna, E., Costantini, A., Fuganti, C., Gatti, F.G., Malpezzi, L., and Serra, S., J. Org. Chem., 2006, vol. 71, no. 14, pp. 5228–5240.
Mandal, D., Ahmad, A., Islam, K.M., and Kumar, R., J. Mol. Catal. B: Enzym., 2004, vol. 27 nos. 2–3, pp. 61–63.
Goswami, A. and Goswami, J., Tetrahedron Lett., 2005, vol. 46, no. 25, pp. 4411–4413.
Suginaka, K., Hayashi, Y., and Yamamoto, Y., Tetrahedron: Asyrametry, 1996, vol. 27, no. 35, pp.1153–1158.
Ivanov, A.E. and Schneider, M.P., J. Mol. Catal. B: Enzym., 1997, vol. 3, no. 6, pp. 303–309.
Hoffmann, I., Silva, V.D., and Nascimento, M.D.G., J. Braz. Chem. Soc., 2011, vol. 22, no. 8, pp. 1559–1567.
Faraldos, J.A., Giner, J., Smith, D.H., Wilson, M., Ronhovde, K., Wilson, E., et al., J. Chem. Educ., 2011, vol. 88, no. 3, pp. 334–336.
Farbood, M.I., Blocker, R.W., Arvizzigno, J., and Muralidhara R., US Patent no. US 6602535 B1, 2003.
Chua, L.S. and Sarmidi, M.R., J. Mol. Catal. B: Enzym., 2004, vol. 28, nos. 2–3, pp. 111–119.
Yun, H., Yang, Y., Cho, B., Hwang, B., and Kim, B., Biotechnol. Lett., 2003, vol. 25, no. 10, pp. 809–814.
Homola, P., Kurák, T., Illeová, V., and Polakovic, M., Food Bioprod. Process., 2015, vol. 96, pp. 126–132.
Rauter, M., Kasprzak, J., Denter, S., Becker, K., Baronian, K., Bode, R., et al., J. Mol. Catal. B: Enzym., 2014, vol. 108, no. 1, pp. 72–76.
Rauter, M., Kasprzak, J., Becker, K., Baronian, K., Bode, R., Kunze, G., and Vorbrodt, H.M., J. Mol. Catal. B: Enzym., 2014, vol. 104, no. 2, pp. 8–16.
Kasprzak, J., Bischoff, F., Rauter, M., Becker, K., Baronian, K., Bode, R., et al., J. Biochem. Eng., 2016, vol. 106, pp. 107–117.
Li, Y.L., Xu, J.H., and Xu, Y., J. Mol. Catal. B: Enzym., 2010, vol. 64, nos. 1–2, pp. 48–52.
Qian, W.K., Zhang, L., Sun, H.F., and Liu, J.H., Adv. Synth. Catal., 2012, vol. 354, no. 17, pp. 3231–3236.
Hummel, W., Appl. Microbiol. Biotechnol., 1990, vol. 34, no. 1, pp. 15–19.
Hummel, W., Adv. Biochem. Eng. Biotechnol., 1997, vol. 58, no. 4, pp. 145–184.
Cripps, R.E. and Trudgill, P.W., Eur. J. Biochem., 1978, vol. 86, pp. 175–186.
Wang, M. and Yuan, W.Q., Bioresour. Technol., 2015, vol. 193, pp. 171–177.
Han, Y.J. and Chen, H.Z., J. Mol. Catal. B: Enzym., 2010, vol. 63, no. 3–4, pp. 135–140.
Laemmli, U.K., Nature, 1970, vol. 227, no. 5259, pp. 680–685.
Zhou, S., Zhang S.C., Lai, D.Y., Zhang, S.L., and Chen, Z.M., Biotechnol. Lett., 2013, vol. 35, no. 3, pp. 359–365.
Yanai, H., Doi, K., and Ohshima, T., Appl. Environ. Microb., 2009, vol. 75, no. 6, pp. 1758–1763.
Hirakawa, H., Kamiya, N., Kawarabayasgi, Y., and Nagamune, T., J. Biosci. Bioeng., 2004, vol. 97, no. 3, pp. 202–206.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Zhang, X., Yang, J., Yang, C. et al. Purification and Characterization of a Novel (R)-1-Phenylethanol Dehydrogenase from Lysinibacillus sp. NUST506. Appl Biochem Microbiol 54, 149–154 (2018). https://doi.org/10.1134/S0003683818020126
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683818020126