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MicroRNA-630 suppresses epithelial-to-mesenchymal transition by regulating FoxM1 in gastric cancer cells

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Abstract

In the present study, we investigated the functional role of microRNA (miR)-630 in epithelial-to-mesenchymal transition (EMT) of gastric cancer (GC) cells, as well as the regulatory mechanism. Cells of human GC cell line SGC 7901 were transfected with miR-630 mimic or miR-630 inhibitor. The transfection efficiency was confirmed by qRT-PCR. Cell migration and invasion were determined by Transwell assay. Protein expression of E-cadherin, vimentin, and Forkhead box protein M1 (FoxM1) was tested by Western blot. Moreover, the expression of FoxM1 was elevated or suppressed, and then the effects of miR-630 abnormal expression on EMT and properties of migration and invasion were examined again, as well as protein expression of Ras/phosphoinositide 3-kinase (PI3K)/AKT related factors. The results showed that (i) the EMT and properties of migration and invasion were statistically decreased by overexpression of miR-630 compared to the control group but markedly increased by suppression of miR-630. However, (ii) abnormal expression of FoxM1 reversed these effects in GC cells. Moreover, (iii) expression of GTP-Rac1, p-PI3K, and p-AKT was decreased by miR-630 overexpression but increased by FoxM1 overexpression. (iv) The decreased levels of GTP-Rac1, p-PI3K, and p-AKT induced by miR-630 overexpression were dramatically elevated by simultaneous overexpression of FoxM1. In conclusion, our results suggest that miR-630 might be a tumor suppressor in GC cells. MiR-630 suppresses EMT by regulating FoxM1 in GC cells, supposedly via inactivation of the Ras/PI3K/AKT pathway.

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Abbreviations

EMT:

epithelial-to-mesenchymal transition

FoxM1:

Forkhead box protein M1

GC:

gastric cancer (cells)

miR-630:

microRNA-630

PI3K:

phosphoinositide 3-kinase

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Authors and Affiliations

  1. Department of Gastroenterology, The Second Affiliated Hospital of Zhengzhou University, 450014, Zhengzhou, Henan Province, China

    Jing Feng & Changwei Feng

  2. Department of Oncology, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, Henan Province, China

    Xiaojuan Wang

  3. Department of Respiratory Medicine, Zhengzhou YIHE Hospital, 450047, Zhengzhou, Henan Province, China

    Weihua Zhu & Si Chen

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  1. Jing Feng
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  2. Xiaojuan Wang
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  3. Weihua Zhu
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  4. Si Chen
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  5. Changwei Feng
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Correspondence to Changwei Feng.

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Published in Russian in Biokhimiya, 2017, Vol. 82, No. 6, pp. 934-943.

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Feng, J., Wang, X., Zhu, W. et al. MicroRNA-630 suppresses epithelial-to-mesenchymal transition by regulating FoxM1 in gastric cancer cells. Biochemistry Moscow 82, 707–714 (2017). https://doi.org/10.1134/S0006297917060074

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  • DOI: https://doi.org/10.1134/S0006297917060074

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