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Changes of gas exchange parameters and of functional-biochemical properties of erythrocytes in dynamics of experimental anemia in rats

  • Comparative and Ontogenic Physiology
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Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

In experiments on male Wistar rats, in a specially constructed computerized installation, O2 consumption by the animals in comparison with changes of hematological, biochemical, and rheological blood properties is studied after anemization—acute blood loss (12–15% of the total blood mass). An increase of the O2 consumption by the organism and tissues by 18–28% has been revealed for the first 7 days after the blood loss, in spite of a pronounced decrease of hematocrit and of the amount of erythrocytes and hemoglobin in peripheral blood by 20–25% of the initial level. There was a 5–10-fold increase of the content of immature erythrocyte forms—reticulocytes and a progressive rise of cell acidic resistance, which is characteristic of young erythrocyte forms. An increase of O2 consumption at a decrease of the blood oxygen capacity (a low hemoglobin level) seems to be due to the more efficient transport and yield of O2 to tissues. At the 3rd and 7th day after the blood loss, activity of Na,K-ATPase has been found to increase by 60% and 20%, respectively. Analysis of the erythrocyte rheological properties has shown that the maximal firmness of aggregates (Uq) and the aggregation rate (1/T) decrease progressively beginning from 3 days after the blood loss; index of deformability (Imax) turned out to be elevated by 7–11%, probably due to an increase of the cell membrane elasticity. The conclusion is made that changes of erythrocyte rheological properties are interconnected with changes of the Na,K-ATPase activity and are directed at optimization of blood circulation in large vessels and the capillary network.

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Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    M. N. Maslova, A. M. Kazennov, L. N. Katyukhin, A. V. Novozhilov, E. A. Skverchinskaya & T. V. Tavrovskaya

  2. Institute of Analytical Instruments Making, Russian Academy of Sciences, St. Petersburg, Russia

    L. P. Kislyakova & Yu. Ya. Kislyakov

Authors
  1. M. N. Maslova
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  2. L. P. Kislyakova
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  3. A. M. Kazennov
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  4. Yu. Ya. Kislyakov
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  5. L. N. Katyukhin
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  6. A. V. Novozhilov
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  7. E. A. Skverchinskaya
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  8. T. V. Tavrovskaya
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Additional information

Original Russian Text © M. N. Maslova, L. P. Kislyakova, A. M. Kazennov, Yu. Ya. Kislyakov, L. N. Katyukhin, A. V. Novozhilov, E. A. Skverchinskaya, T. V. Tavrovskaya, 2009, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2009, Vol. 45, No. 5, pp. 498–504.

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Maslova, M.N., Kislyakova, L.P., Kazennov, A.M. et al. Changes of gas exchange parameters and of functional-biochemical properties of erythrocytes in dynamics of experimental anemia in rats. J Evol Biochem Phys 45, 604–611 (2009). https://doi.org/10.1134/S0022093009050077

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  • DOI: https://doi.org/10.1134/S0022093009050077

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