Abstract
This study addresses for the first time the seasonal (winter–summer) dynamics of endogenous ethanol (EE) and acetaldehyde (EA) blood levels as well as of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities and catalytic properties in the liver of the reindeer. EE blood level was 1.89 ± 0.11 mM in summer and 1.41 ± 0.10 mM (i.e. 1.34 times lower) in winter (p< 0.05). Seasonal dynamics of EA blood levels was opposite: 3.06 ± 0.28 μM in summer and 12.62 ± 0.76 μM (i.e. 4.12 times higher) in winter (p< 0.05). In winter, there was a decrease in activities of ADHII in the acetaldehyde reduction reaction (4.0 times) and ALDH (3.3 times) in the liver (p< 0.05). This led to an increased winter blood level of EA in cold-adapted animals. Our results suggest that EE and EA, as interrelated metabolites, make up one of the systems that regulate the metabolic rate and eventually reduce the intensity of energy metabolism in the reindeer. This consi derably extends the possibilities for northern species to exist under extreme environmental conditions of cold regions. Apparently, a major ph ysiological function of the system that includes EE, EA and relevant enzymes is to increase cold tolerance of an organism due to the inhibitory effect of EA on mitochondrial terminal oxidation which reduces the intensity of aerobic processes.
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References
Khaskin, V.V., Energetika teploobrazovaniya i adaptatsii k kholodu (Energetics of Heat Production and Cold Adaptation), Novosibirsk, 1975.
Anufriev, A.I., Ekologicheskie mekhanizmy temperaturnykh adaptatsyi mlekopitayushchikh i zimuyushchikh ptits Yakutii (Ecological Mechanisms of Thermal Adaptations in Mammals and Wintering Birds of Yakutia), Novosibirsk, 2013, pp. 172–177.
Slonim, A.D., Termicheskie adaptatsii i temperaturnye kompensatsii u gomoiotermnykh zhivotnykh, Fiziologiya zhivotnykh (Thermal Adaptations and Temperature Compensations in Homeothermic Organisms, Animal Physiology), Leningrad, 1981, pt. 3, pp. 41–47.
Cheremnykh, I.E., Roshchevsky, M.P., and Novozhilova, E.A., Kopytnye zhivotnye v usloviyakh Severa (Ungulates in the North), Leningrad, 1980.
Kolosova, O.N., Kirshengolts, B.M., and Solomonov, N.G., Endogenous ethanol and acetaldehyde in regulatory mechanisms of vital activity of the organism during hibernation, Dokl. RAN, 2000, vol. 441, no. 1, pp. 118–121.
Kolosova, O.N. and Kershengolts, B.M., Stabilization of homeostasis in rats during cold exposures with ethanol, Bull. Exp. Biol. Med., 2016, vol. 160, pp. 300–303.
Kolosova, O.N. and Kershengolts, B.M., Condition of ethanol/acetaldehyde system and its role in resistance to alcoholization in populations of northern people, Ekol. Chel., 2015, no. 6, pp. 24–32.
Jone Jornvall, H., Hoog, J., Bahr-Lindstrom, H., et al., Alcohol dehydrogenases and aldehyde dehydrogenases, Biochem. Soc. Trans., 1988, vol. 6, no. 3, pp. 223–227.
Pontes, H., Pinho, P.G., Casal, S., Carmo, H., Santos, A., Magalhães, T., Remião, F., Carvalho, F., and Bastos, M.L., GC determination of acetone, acetaldehyde, ethanol and methanol in biological matrices and cell culture, J. Chromatogr. Sci., 2009, vol. 47, pp. 272–278.
Rogozhin, V.V., Govorova, T.P., and Kershengolts, B.M., Selective method for titration of alcohol dehydrogenase active centers by chloro-phydroxymercuribenzoate, Bioorg. Khim., 1988, vol. 14, no. 12, pp. 16–26.
Kershengolts, B.M. and Serkina, E.V., Nekotorye metodicheskie podkhody k izucheniyu metabolizma etanola (Some Methodological Approaches to Studying Ethanol Metabolism), Moscow, 1981, VINITI, no. D-3793.
Kershengolts, B.M. and Turnin, Kh.Kh., Polymorphism of ADH and ALDH in groups of Yakutia population and forecast of population resistance to alcohol, Uch. Zap. Yakut. Gosuniver., 1994, pp. 138–149.
Glanz, S., Mediko-biologicheskaya statistica (Medico-Biological Statistics), Moscow, 1998, pp. 51–196.
Chernobrovkina, T.V. and Kershengolts, B.M., Fundamental’nye i mediko-sotsial’nye aspekty addiktologii (Fundamental and Medico-Social Aspects of Addictology), Yakutsk, 2011, vol.1.
Agarwal, D.P. and Seitz, H.K., Alcohol in Health and Disease, New York, Basel, 2001.
Kolosova, O.N., Ecologo-physiological mechanisms of metabolism regulation in adaptation of higher vertebrates to northern conditions, Doctorate Sci. Diss., Moscow, 1998.
Sokolov, A.Ya., Ecological energetics of the reindeer, Doctorate Sci. Diss., Vladivostok, 1999.
Khlenyy, E.S., Kolosova, O.N., Anufriev, A.I, and Kerschengolts, B.M., Experimental modelling of hipotermia in animals by means of modified regulator peptides, extracted from hibernating animals, Cryobiol., 2010, vol. 61, no. 3, pp. 365–366.
Kramarova, L.I., Ziganshin, R.Kh., and Gakhova, E.N., Endogenous hypometabolic–hypothermic factors and their possible application to life in the cold, Russ. J. Bioorg. Chem., 2009, vol. 35, no. 5, pp. 538–548.
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Original Russian Text © O.N. Kolosova, B.M. Kershengolts, 2018, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2018, Vol. 54, No. 2, pp. 91–95.
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Kolosova, O.N., Kershengolts, B.M. Physiological Functions and Metabolism of Endogenous Ethanol and Acetaldehyde in the Reindeer. J Evol Biochem Phys 54, 103–108 (2018). https://doi.org/10.1134/S0022093018020023
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DOI: https://doi.org/10.1134/S0022093018020023