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Constant urinary proteins in healthy humans in a 520-day isolation experiment

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Abstract

The aim of this study was the search of permanent proteins of the urinary proteome during a 520-day isolation experiment at the Institute of Biomedical Problems (IBMP) Ground-Based Test Facility in controlled conditions, using an autonomous life support system. The object of the study was urine sampled from 6 normal male subjects aged 25 to 37. The biological material samples (the second morning urine fractions) were collected for proteomic investigations against the background, on the 50th, 93rd, 124th, 153rd, 180th, 251st, 274th, 303rd, 330th, 371st, 400th, and 427th days of isolation, and on the 7th day after its completion. The samples were analyzed using chromatography–mass spectrometry, while the obtained results were analyzed using bioinformatics resources. The following seven permanent proteins were identified and observed during the entire period of urine investigations: epidermal growth factor, polymeric immunoglobulin receptor, plasma serine protease inhibitor, Alpha 1 microglobulin/bikunin precursor (AMBP), keratin (type II cytoskeletal 1), collagen alpha-1 (VI) chain, and serum albumin.

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References

  1. Agron, I.A., Avtonomov, D.M., Kononikhin, A.S., et al., Accurate mass tag retention time database for urine proteome analysis by chromatography-mass spectrometry, Biochemistry, 2010, vol. 75, no. 5, pp. 636.

    CAS  PubMed  Google Scholar 

  2. Babichenko, I.I. and Kovyazin, V.A., Novye metody immunogistokhimicheskoi diagnostiki opukholevogo rosta (New Methods of Tumor Growth by Immunohistochemical Diagnostics), Moscow, 2008.

    Google Scholar 

  3. Adachi, J., Kumar, C., Zhang, Y., et al., The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins, Genome Biol., 2006, vol. 7, no. 9, p. R80.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Albalat, A., Mischak, H., and Mullen, W., Urine proteomics in clinical applications: technologies, principal considerations and clinical implementation, Prilozi, 2011, vol. 32, no. 1, pp. 13.

    CAS  PubMed  Google Scholar 

  5. Allhorn, M., Berggård, T., Nordberg, J., et al., Processing of the lipocalin alpha(1)-microglobulin by hemoglobin induces heme-binding and heme-degradation properties, Blood, 2002, vol. 15, no. 99, pp. 1894.

    Article  Google Scholar 

  6. Christensen, E.I. and Gburek, J., Protein reabsorption in renal proximal tubule-function and dysfunction in kidney pathophysiology, Pediatr. Nephrol., 2004, vol. 9, no. 7, pp. 714.

    Article  Google Scholar 

  7. Court, M., Selevsek, N., Matondo, M., et al., Toward a standardized urine proteome analysis methodology, Proteomics, 2011, vol. 11, no. 6, pp. 1160.

    Article  CAS  PubMed  Google Scholar 

  8. España, F., Navarro, S., Medina, P., et al., The role of protein C inhibitor in human reproduction, Semin. Thromb. Hemostasis, 2007, vol. 33, no. 1, pp. 41.

    Article  Google Scholar 

  9. Glogowska, A., Stetefeld, J., Weber, E., et al., Epidermal growth factor cytoplasmic domain affects ErbB protein degradation by the lysosomal and ubiquitinproteasome pathway in human cancer cells, Neoplasia, 2012, vol. 14, no. 5, pp. 396.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Hamburger, A.E., West, A.P.Jr., and Bjorkman, P.J., Crystal structure of a polymeric immunoglobulin binding fragment of the human polymeric immunoglobulin receptor, Structure, 2004, vol. 12, no. 11, pp. 1925.

    Article  CAS  PubMed  Google Scholar 

  11. Igci, M., Arslan, A., Igci, Y.Z., et al., Bikunin and a1-microglobulin/bikunin precursor (AMBP) gene mutational screening in patients with kidney stones: a casecontrol study, Scand. J. Urol. Nephrol., 2010, vol. 44, no. 6, pp. 413.

    Article  CAS  PubMed  Google Scholar 

  12. Ishihama, Y., Rappsilber, J., Andersen, J.S., and Mann, M., Microcolumns with self-assembled particle frits for proteomics, J. Chromatogr., A, 2002, vol. 979, nos. 1–2, p. 233.

    Article  CAS  Google Scholar 

  13. Ishikawa, H., Sugie, K., Murayama, K., et al., Ullrich disease due to deficiency of collagen VI in the sarcolemma, Neurology, 2004, vol. 62, no. 4, pp. 620.

    Article  CAS  PubMed  Google Scholar 

  14. Kuritzky, L., Toto, R., and Van Buren, P., Identification and management of albuminuria in the primary care setting, J. Clin. Hypertens., 2011, vol. 13, no. 6, pp. 438.

    Article  CAS  Google Scholar 

  15. Lampe, A.K. and Bushby, K.M., Collagen VI related muscle disorders, J. Med. Genet., 2005, vol. 42, no. 9, pp. 673.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Lei, T., Zhao, X., Jin, S., et al., Discovery of potential bladder cancer biomarkers by comparative urine proteomics and analysis, Clin. Genitourin. Cancer, 2013, vol. 11, no. 1, pp. 56.

    Article  PubMed  Google Scholar 

  17. Malmström, E., Mörgelin, M., Malmsten, M., et al., Protein c inhibitor—a novel antimicrobial agent, PLoS Pathog., 2009, vol. 5, no. 12, p. e1000698.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Nagaraj, N. and Mann, M., Quantitative analysis of the intra-and inter-individual variability of the normal urinary proteome, J. Proteome Res., 2011, vol. 10, no. 2, pp. 637.

    Article  CAS  PubMed  Google Scholar 

  19. Rouet, P., Raguenez, G., Ruminy, P., and Salier, J.P., An array of binding sites for hepatocyte nuclear factor 4 of high and low affinities modulates the liver-specific enhancer for the human alpha1-microglobulin/bikunin precursor, Biochem. J., 1998, vol. 15, no. 334, pp. 577.

    Article  Google Scholar 

  20. Schweizer, J., Bowden, P.E., Coulombe, P.A., et al., New consensus nomenclature for mammalian keratins, J. Cell Biol., 2006, vol. 174, no. 2, pp. 169.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Tan, Y., Zhang, J.J., Liu, G., et al., The level of urinary secretory immunoglobulin A (sIgA) of patients with IgA nephropathy is elevated and associated with pathological phenotypes, Clin. Exp. Immunol., 2009, vol. 156, no. 1, pp. 111.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Thulesen, J., Jørgensen, P.E., Torffvit, O., et al., Urinary excretion of epidermal growth factor and Tamm-Horsfall protein in three rat models with increased renal excretion of urine, Regul. Pept., 1997, vol. 72, nos. 2–3, p. 179.

    Article  CAS  PubMed  Google Scholar 

  23. Tojo, A. and Kinugasa, S., Mechanisms of glomerular albumin filtration and tubular reabsorption, Int. J. Nephrol., 2012. http://www.hindawi.com/journals/ijn/2012/481520/.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    L. Kh. Pastushkova, K. S. Kireev, I. V. Dobrokhotov & I. M. Larina

  2. CaDyWEC Associated Russian–French Laboratory, Moscow, Russia

    L. Kh. Pastushkova, I. V. Dobrokhotov, M. -A. Custaud & I. M. Larina

  3. Gagarin Research and Testing Cosmonaut Training Center, Moscow oblast, Russia

    K. S. Kireev & V. I. Pochuev

  4. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia

    A. S. Kononikhin, I. A. Popov & E. N. Nikolaev

  5. Institute of Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

    A. S. Kononikhin, I. A. Popov & E. N. Nikolaev

  6. University of Angers, Angers, France

    M. -A. Custaud

  7. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    E. S. Tiys, V. A. Ivanisenko & N. A. Kolchanov

  8. CaDyWEC Associated Russian–French Laboratory, Angers, France

    L. Kh. Pastushkova, I. V. Dobrokhotov, M. -A. Custaud & I. M. Larina

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  1. L. Kh. Pastushkova
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  2. K. S. Kireev
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  3. A. S. Kononikhin
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  4. E. S. Tiys
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  5. I. A. Popov
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  6. I. V. Dobrokhotov
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  7. M. -A. Custaud
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  8. V. A. Ivanisenko
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  9. N. A. Kolchanov
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  10. E. N. Nikolaev
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  11. V. I. Pochuev
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  12. I. M. Larina
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Corresponding author

Correspondence to I. M. Larina.

Additional information

Original Russian Text © L.Kh. Pastushkova, K.S. Kireev, A.S. Kononikhin, E.S. Tiys, I.A. Popov, I.V. Dobrokhotov, M.-A. Custaud, V.A. Ivanisenko, N.A. Kolchanov, E.N. Nikolaev, V.I. Pochuev, I.M. Larina, 2014, published in Aviakosmicheskaya i Ekologicheskaya Meditsina, 2014, Vol. 48, No. 1, pp. 48–54.

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Pastushkova, L.K., Kireev, K.S., Kononikhin, A.S. et al. Constant urinary proteins in healthy humans in a 520-day isolation experiment. Hum Physiol 42, 760–765 (2016). https://doi.org/10.1134/S0362119716070136

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  • DOI: https://doi.org/10.1134/S0362119716070136

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