Abstract
Fluorochrome-labeled oligonucleotides (n = 44) corresponding to mouse genome repetitive sequences were hybridized in situ with pachytene nuclei of mouse spermatocytes. Signals of the repetitive sequences MaLR, MER, and (GT)22 were found to be dispersed through chromatin, and signals of B1 repeats and minisatellites were mostly attached to synaptonemal complexes immunostained with anti-SYCP3 antibodies. These results suggest that B1 repeats and minisatellites are candidates for sequences anchoring chromatin to synaptonemal complexes.
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Karpova, O.I., Safronov, V.V., Zaitseva, S.P., and Bogdanov, Yu.F., Characteristics of DNA from Isolated Fraction of Mouse Synaptonemal Complexes, Mol. Biol. (Moscow), 1989, vol. 23, no. 2, pp. 571–579.
Karpova, O.I., Penkina, M.V., Dadashev, S.Ya., et al., Specific Features of Primary DNA Structure from Golden Hamster Synaptonemal Complex, Mol. Biol. (Moscow), 1995, vol. 29, no. 3, pp. 512–521.
Pearlman, R.E., Tsao, N., and Moens, P.B., Synaptonemal Complexes from DNase-Treated Rat Pachytene Chromosomes Contain (GT)n and LINE/SINE Sequences, Genetics, 1992, vol. 130, no. 4, pp. 865–872.
Hernández-Hernández, A., Rincón-Arano, H., Recillas-Targa, F., et al., Differential Distribution and Association of Repeat DNA Sequences in the Lateral Element of the Synaptonemal Complex in Rat Spermatocytes, Chromosoma, 2008, vol. 117, no. 1, pp. 77–87.
Zickler, D. and Kleckner, N., Meiotic Chromosomes: Integrating Structure and Function, Ann. Rev. Genet., 1999, vol. 33, pp. 603–754.
Penkina, M.V., Karpova, O.I., Zakharevich, N.V., et al., SCAR-DNA Family Is Enriched with Conserved Sequences, Mol. Biol. (Moscow), 2008, vol. 42, no. 2, pp. 362–369.
Kolomiets, O.L., Abuduev, N.K., Mazurova, T.F, et al., Damaging Effect of Antibiotics on the Structure of Synaptonemal Complexes of Meiotic Mouse, Russ. J. Genet., 2001, vol. 37, no. 2, pp. 197–206.
Dadashev, S.Ya., Grishaeva, T.M., and Bogdanov, Yu.F., In silico Identification and Characteristic of the Sequences of Meiotic DNA: AluJb Possibly Participates in the Attachment of Chromatin Loops to Synaptonemal Complex, Russ. J. Genet., 2005, vol. 41, no. 12, pp. 1707–1713.
Dadashev, S.Ya., Bashkirov, V.I., Belostotskii, D.I., et al., DNA Specific Features from the Fraction of Isolated Mouse Synaptonemal Complexes, Dokl. Akad. Nauk SSSR, 1989, vol. 308, no. 2, pp. 490–493.
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Original Russian Text © V.E. Spangenberg, S.Ya. Dadashev, S.N. Matveevsky, O.L. Kolomiets, Yu.F. Bogdanov, 2010, published in Genetika, 2010, Vol. 46, No. 10, pp. 1363–1366.
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Spangenberg, V.E., Dadashev, S.Y., Matveevsky, S.N. et al. How do chromosomes attach to synaptonemal complexes?. Russ J Genet 46, 1203–1205 (2010). https://doi.org/10.1134/S1022795410100157
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DOI: https://doi.org/10.1134/S1022795410100157