Abstract
Structural and functional organization of genes responsible for biosynthesis of amino acid methionine, which plays a leading role in cellular metabolism of bacteria, was studied in 24 natural Yersinia pestis strains of the major and non-main subspecies from various natural plague foci located in the territory of Russian Federation and neighbouring foreign countries, and also in Y. pestis and Y. pseudotuberculosis strains recorded in the files of NCBI GenBank database. Conservatism of genes metA, metC, metE, and metH as well as regulatory genes metR and metJ involved in biosynthesis of this amino acid was established. Sequencing of the variable locus of gene metB in natural Y. pestis strains of major and non-main subspecies revealed that the reason for the methionine dependence of strains belonging to the main subspecies is a deletion of a single nucleotide (−G) in the 988 position from the beginning of the gene, whereas this dependence in strains belonging to subspecies hissarica results from the appearance of a single nucleotide (+G) insertion in the 989 position of gene metB. These mutations are absent in strains of the caucasica, altaica, and ulegeica subspecies of the plague agent and in strains of pseudotuberculosis microbe, which correlates with their capacity for methionine biosynthesis.
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Onishchenko, G.G., Kutyrev, V.V., Popov, N.V., et al., Prirodnye ochagi chumy Kavkaza, Prikaspiya, Srednei Azii i Sibiri (Natural Foci of Plague in the Caucasus, Ciscaspian Area, Central Asia, and Siberia), Moscow: Meditsina, 2004.
Kutyrev, V.V., Konnov, N.P., and Volkov, Yu.P., Vozbuditel’ chumy: ul’trastruktura i lokalizatsiya v perenoschike (The Cause of Plague: Ultrastructure and Localization in a Vector), Moscow: Meditsina, 2007.
Achtman, M., Morelli, G., Zhou, P., et al., Yersinia pestis, the Cause of Plague, Is a Recently Emerged Clone of Yersinia pseudotuberculosis, Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 14043–14048.
Parkhill, J., Wren, B., Thomson, N., et al., Genome Sequence of Yersinia pestis, the Causative Agent of Plague, Nature, 2001, vol. 413, pp. 523–527.
Wren, B.W., The Yersinia—a Model Genus to Study the Rapid Evolution of Bacterial Pathogen, Nat. Rev. Microbiol., 2003, vol. 1, pp. 55–64.
Chain, P.S.G., Carniel, E., Larimer, F.W., et al., Insights into the Evolution of Yersinia pestis through Whole Genome Comparison with Yersinia pseudotuberculosis, Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 13822–13832.
Brubaker, R.R., The Recent Emergence of Plague: A Process of Felonious Evolution, Microbial Ecol., 2004, vol. 47, pp. 293–299.
Kukleva, L.M., Protsenko, O.A., and Kutyrev, V.V., Modern Concepts on the Relationship between the Agents Causing Plague and Pseudotuberculosis, Mol. Genet. Mikrobiol. Virusol., 2002, no. 1, pp. 3–7.
Kutyrev, V.V., Eroshenko, G.A., Popov, N.V., et al., Molecular Mechanisms of Interactions of Plague Causative Agents with Invertebrates, Mol. Genet., Mikrobiol. Virusol., 2009, no. 4, pp. 6–13.
Kukleva, L.M., Eroshenko, G.A., Shavina, N.Yu., et al., Comparative Analysis of Distribution of Pseudogenes in the Genome of Strains of Basic and Supplementary Subspecies of the Plague Infection Agent, Mol. Genet., Mikrobiol. Virusol., 2009, no. 2, pp. 32–36.
Eroshenko, G.A., Vidyaeva, N.A., Odinokov, G.N., et al., Structural and Functional Analysis of the araN Gene in the Yersinia pestis Strains of Different Origin, Mol. Genet., Mikrobiol. Virusol., 2009, no. 3, pp. 21–26.
Auger, S., Yuen, W.H., and Danchin, A., The metIC Operon Involved in Methionine Biosynthesis in Bacillus subtilis Is Controlled by Transcription Antitermination, J. Microbiol., 2002, vol. 148, pp. 507–518.
Martinevskii, I.L., Stepanov, V.M., and Kenzhebaev, A.Ya., Taksonomiya, mutatsiya i genetika patogennosti chumnogo i rodstvennykh emu mikrobov (Taxonomy, Mutation and Genetics of Pathogenicity of Plague and Related Microbes), Nukus, 1990.
Sultanov, G.V. and Kozlov, M.P., Chuma: Microbiologia, Pathogenes, Diagnostika (Plague: Microbiology, Pathogenesis, and Diagnostics), Makhachkala: Izd. Akad. S-kh. Nauk, 1995, vol. 1.
Sludskii, A.A., Derlyatko, K.I., Golovko, E.N., and Ageev, V.S., Gissarskii prirodnyi ochag chumy (Hissar Natural Focus of Plague), Saratov: Izd. Saratov Univ., 2003.
Aparin, G.P. and Golubinskii, E.P., Mikrobiologiya chumy: Rukovodstvo (Microbiology of Plague: The Guide), Irkutsk: Izd. Irkutsk Unv., 1989.
Sanger, F., Nicklen, S., and Coulson, A.R., DNA Sequencing with Chain-Terminating Inhibitors, Proc. Natl. Acad. Sci. USA, 1977, vol. 74, pp. 5463–5467.
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Original Russian Text © G.N. Odinokov, G.A. Eroshenko, Ya.M. Krasnov, L.M. Koukleva, N.Yu. Shavina, A.I. Pavlova, V.V. Kutyrev, 2011, published in Genetika, 2011, Vol. 47, No. 3, pp. 332–338.
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Odinokov, G.N., Eroshenko, G.A., Krasnov, Y.M. et al. Genetic bases of methionine dependence in Yersinia pestis strains of main and non-main subspecies. Russ J Genet 47, 289–294 (2011). https://doi.org/10.1134/S102279541101008X
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DOI: https://doi.org/10.1134/S102279541101008X